Iwamori Tokuko, Nukumi Naoko, Itoh Kikuji, Kano Kiyoshi, Naito Kunihiko, Kurohmaru Masamichi, Yamanouchi Keitaro, Tojo Hideaki
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
J Vet Med Sci. 2010 May;72(5):621-5. doi: 10.1292/jvms.08-0331. Epub 2009 Dec 15.
We have previously reported the action of whey acidic protein (WAP) inhibiting the proliferation of mouse mammary epithelial cells in the experiments utilizing in vivo and in vitro systems. We report herein the bacteriostatic activity of WAP. Western blot analysis demonstrated successful isolation of WAP from whey fractions of rat milk by column chromatography. The WAP fraction inhibited the growth of Staphylococcus aureus JCM2413 in a dose-dependent manner, but did not inhibit the growth of Escherichia coli. The bacteriostatic activity of WAP was highest at pH 6.6 and was not affected by the presence of 150 mM NaCl. A scanning electron micrograph of bacteria treated with WAP exhibited the disruption of the bacterial cell walls.
我们之前曾报道过，在利用体内和体外系统进行的实验中，乳清酸性蛋白（WAP）具有抑制小鼠乳腺上皮细胞增殖的作用。在此我们报告WAP的抑菌活性。蛋白质免疫印迹分析表明，通过柱色谱法成功从大鼠乳汁的乳清组分中分离出了WAP。WAP组分以剂量依赖性方式抑制金黄色葡萄球菌JCM2413的生长，但不抑制大肠杆菌的生长。WAP的抑菌活性在pH 6.6时最高，且不受150 mM NaCl存在的影响。用WAP处理的细菌的扫描电子显微镜照片显示细菌细胞壁被破坏。
