Department of Clinical Immunology, Medical University, Wrocław, Poland.
Int J Gynecol Cancer. 2009 Nov;19(8):1322-8. doi: 10.1111/IGC.0b013e3181b70465.
The modification of p53 protein by phosphorylation plays an important role in its stabilization and the regulation of its biological properties. The study investigated the expression of p53 protein phosphorylated at serine 20 (Ser20) and Ser392 and the association between clinicopathological parameters of ovarian neoplasms with respect to p53 protein overexpression.
p53 protein expression was evaluated on tissues from malignant and benign ovarian tumors. Protein expression was measured in a subset of the specimens using immunohistochemistry.
The correlation between p53 protein overexpression and p53-Ser392 phosphorylation was found in ovarian carcinomas (P = 0.001, r = +0.27). In the total group of ovarian carcinomas, significant differences were observed in p53 protein overexpression between well (G1) and poor (G3) tumor grades (P = 0.005) and between serous and endometrioid types of tumor (P = 0.04), whereas p53-Ser20 phosphorylation was associated with advanced International Federation of Gynecology and Obstetrics stage (P = 0.004) and high tumor grade (P = 0.02). In p53-positive ovarian carcinomas, p53-Ser392 phosphorylation was associated with advanced tumor stage (P = 0.02) and high tumor grade (P = 0.049). p53-Ser20 phosphorylation was associated with low tumor grade of p53-positive ovarian carcinomas (P = 0.02) and with high tumor grade of p53-negative ovarian carcinomas (P = 0.02).
These results revealed that p53 phosphorylation at Ser20 and Ser392 is an early event in ovarian tumor development. The authors suggest that the expression of p53 protein phosphorylated at Ser20 and Ser392 in ovarian carcinomas determines their individual clinical features depending on p53 protein status and may be useful biological biomarkers characterizing their behavior.
磷酸化修饰的 p53 蛋白在其稳定性和生物特性调节中起着重要作用。本研究调查了丝氨酸 20 （Ser20）和 Ser392 磷酸化的 p53 蛋白的表达，并研究了卵巢肿瘤的临床病理参数与 p53 蛋白过表达之间的关系。
评估了恶性和良性卵巢肿瘤组织中的 p53 蛋白表达。使用免疫组织化学法测量了部分标本中的蛋白表达。
在卵巢癌中发现 p53 蛋白过表达与 p53-Ser392 磷酸化之间存在相关性（P = 0.001，r = +0.27）。在卵巢癌的总组中，在良好（G1）和不良（G3）肿瘤分级之间观察到 p53 蛋白过表达的显著差异（P = 0.005）和浆液性和子宫内膜样肿瘤类型之间的差异（P = 0.04），而 p53-Ser20 磷酸化与国际妇产科联合会（FIGO）晚期分期（P = 0.004）和高肿瘤分级相关（P = 0.02）。在 p53 阳性的卵巢癌中，p53-Ser392 磷酸化与晚期肿瘤分期（P = 0.02）和高肿瘤分级（P = 0.049）相关。p53-Ser20 磷酸化与 p53 阳性卵巢癌的低肿瘤分级（P = 0.02）和 p53 阴性卵巢癌的高肿瘤分级（P = 0.02）相关。
这些结果表明，p53 在 Ser20 和 Ser392 处的磷酸化是卵巢肿瘤发展的早期事件。作者认为，卵巢癌中 p53 蛋白在 Ser20 和 Ser392 处的磷酸化表达取决于 p53 蛋白状态，决定了它们各自的临床特征，并可能是其行为的有用生物标志物。
