Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.
Int J Gynecol Cancer. 2009 Nov;19(8):1347-52. doi: 10.1111/IGC.0b013e3181a83f1d.
Human homeobox gene (HOX) A10 is a homeobox allotype gene of the HOXA family in the HOX family. Human homeobox gene A10 may play an important role in cancer development. However, the role of HOXA10 in the carcinogenesis of ovarian clear cell adenocarcinoma (OCCA) has not been established. We have evaluated the prognostic significance of HOXA10 expression for human OCCA and the effects of HOXA10 on proliferation, motility, and invasion of OCCA cells. We found that HOXA10 was not expressed in normal ovarian epithelium, ovarian endometrial cysts, and ovarian serous carcinomas, but 20 (68.9%) of the 29 OCCAs were positive for the expression of HOXA10. Human homeobox gene A10 expression was negatively correlated to the 5-year survival of OCCA patients (R = -0.442, P = 0.043). When a HOXA10 expression vector was stably transfected into a human OCCA cell line, ES-2, the proliferation rate of ES-2-HOXA10 was much higher than the vector control, the motility of ES-2-HOXA10 cells was significantly increased compared with the control (P < 0.05), and the invasion of ES-2-HOXA10 cells was also much higher than the vector control (P < 0.01 at 5 hours and 12 hours after scratching). In conclusion, HOXA10 was overexpressed in OCCA and was correlated with poor survival. HOXA10 promotes proliferation, migration, and invasion of OCCA cells. Human homeobox gene A10 could be a promising prognostic marker for OCCA.
人类同源盒基因（HOX）A10 是 HOX 家族 HOXA 家族的同源盒异型基因。人类同源盒基因 A10 可能在癌症发展中发挥重要作用。然而，HOXA10 在卵巢透明细胞腺癌（OCCA）发生中的作用尚未确定。我们评估了 HOXA10 表达对人 OCCA 的预后意义，以及 HOXA10 对 OCCA 细胞增殖、运动和侵袭的影响。我们发现 HOXA10 在正常卵巢上皮、卵巢子宫内膜囊肿和卵巢浆液性癌中均不表达，但 29 例 OCCAs 中有 20 例（68.9%）表达 HOXA10。人类同源盒基因 A10 表达与 OCCA 患者的 5 年生存率呈负相关（R = -0.442，P = 0.043）。当稳定转染 HOXA10 表达载体至人 OCCA 细胞系 ES-2 中时，ES-2-HOXA10 的增殖率明显高于载体对照，ES-2-HOXA10 细胞的运动能力显著增强（与对照组相比，P < 0.05），并且 ES-2-HOXA10 细胞的侵袭能力也明显高于载体对照（划痕后 5 小时和 12 小时，P < 0.01）。总之，OCCA 中 HOXA10 过表达，与生存不良相关。HOXA10 促进 OCCA 细胞的增殖、迁移和侵袭。人类同源盒基因 A10 可能是 OCCA 有前途的预后标志物。
