Gedik Hale, Ozkan Yasemin Kulak
Ministry of Health, Oral and Dental Health Center, Istanbul, Turkey.
Oral Health Prev Dent. 2009;7(4):347-53.
The objective of the present study was to evaluate surface roughness and adherence of Candida albicans and to determine the effectiveness of denture cleansers in the disinfection of silicone-based soft lining materials.
Two types of silicone-based soft lining materials (four room temperature polymerised soft lining materials [RTPSLM]: Ufi Gel P, Ufi Gel C, Mollosil and Soft-Liner; and two high temperature polymerised soft lining materials [HTPSLM]: Moloplast B and Luci Soft) and seven denture cleansers (Efferdent, Polident, Steradent, Corega, Denclen, Klorhex and Axion) were tested in the present study. For each soft lining material, 56 samples (10 mm x 10 mm x 3 mm) were prepared. Surface roughness measurements were made using a profilometer with an optical scanner probe. All samples were ultrasonically cleaned in water for 15 s, autoclave sterilised and contaminated with C. albicans solutions for adherence assay evaluation. After the evaluation, the samples were immersed in seven different denture cleansers. The results were expressed as the ratio of cells remaining after each cleanser in comparison with the control group.
HTPSLM showed a lower surface roughness value (2.21 +/- 0.30 lm) than RTPSLM (2.39 +/- 0.40 lm), but these differences were not statistically significant (P > 0.05). Also, HTPSLM showed lower adhesion of C. albicans (mean ranks 136.35) than RTPSLM (mean ranks 138.64), but the differences were not statistically significant (P > 0.05, one- or two-way analysis of variance). In the C. albicans removal test, all denture cleansers showed a strong effect on both silicone-based soft lining materials. HTPSLM showed lower removal activity (mean ranks 30.01) than RTPSLM (mean ranks 25.86), but these differences were not statistically significant (P > 0.05). Although there were no significant differences between disinfectants on both silicone-based soft lining materials, Axion was found to be the most effective disinfectant. Corega, Polident, Denclen, Steradent and Efferdent showed the most effective cleansing, respectively.
RTPSLM and HTPSLM did not show significant differences in the surface roughness value; also they showed no significant differences in the adhesion rate of C. albicans. All tested silicone-based soft lining materials showed adherence of C. albicans after contamination procedures, and removal activity for C. albicans was observed in all tested silicone-based soft lining materials after disinfection procedures.
本研究的目的是评估白色念珠菌的表面粗糙度和黏附情况，并确定义齿清洁剂对硅基软衬材料的消毒效果。
本研究测试了两种类型的硅基软衬材料（四种室温聚合软衬材料[RTPSLM]：Ufi Gel P、Ufi Gel C、Mollosil和Soft-Liner；以及两种高温聚合软衬材料[HTPSLM]：Moloplast B和Luci Soft）和七种义齿清洁剂（Efferdent、Polident、Steradent、Corega、Denclen、Klorhex和Axion）。对于每种软衬材料，制备了56个样本（10毫米×10毫米×3毫米）。使用带有光学扫描探头的轮廓仪进行表面粗糙度测量。所有样本在水中超声清洗15秒，高压灭菌并接种白色念珠菌溶液以评估黏附情况。评估后，将样本浸入七种不同的义齿清洁剂中。结果以每种清洁剂处理后剩余细胞与对照组的比例表示。
HTPSLM的表面粗糙度值（2.21±0.30微米）低于RTPSLM（2.39±0.40微米），但这些差异无统计学意义（P>0.05）。此外，HTPSLM上白色念珠菌的黏附性（平均秩次136.35）低于RTPSLM（平均秩次138.64），但差异无统计学意义（P>0.05，单因素或双因素方差分析）。在白色念珠菌清除试验中，所有义齿清洁剂对两种硅基软衬材料均显示出较强的效果。HTPSLM的清除活性（平均秩次30.01）低于RTPSLM（平均秩次25.86），但这些差异无统计学意义（P>0.05）。尽管两种硅基软衬材料上消毒剂之间无显著差异，但Axion被发现是最有效的消毒剂。Corega、Polident、Denclen、Steradent和Efferdent分别显示出最有效的清洁效果。
RTPSLM和HTPSLM在表面粗糙度值上无显著差异；在白色念珠菌的黏附率上也无显著差异。所有测试的硅基软衬材料在污染程序后均显示出白色念珠菌的黏附，在消毒程序后所有测试的硅基软衬材料中均观察到白色念珠菌的清除活性。
