甘氨酸429是L-亮氨酸对人生殖细胞碱性磷酸酶非竞争性抑制作用的主要决定因素。
Gly429 is the major determinant of uncompetitive inhibition of human germ cell alkaline phosphatase by L-leucine.
作者信息
Hummer C, Millán J L
机构信息
La Jolla Cancer Research Foundation, CA 92037.
出版信息
Biochem J. 1991 Feb 15;274 ( Pt 1)(Pt 1):91-5. doi: 10.1042/bj2740091.
Abstract
The catalytic activity of human placental alkaline phosphatase (PLAP) and germ cell alkaline phosphatase (GCAP) can be inhibited, through an uncompetitive mechanism, by L-Phe. GCAP is also selectively inhibited by L-Leu. Site-directed mutagenesis of five of the 12 residues which are different in PLAP and GCAP revealed that Gly429 is the primary determinant of GCAP inhibition by L-Leu, and Ser84 and Leu297 play a modulatory role in the inhibition.
摘要
人胎盘碱性磷酸酶（PLAP）和生殖细胞碱性磷酸酶（GCAP）的催化活性可通过非竞争性机制被L-苯丙氨酸抑制。GCAP还可被L-亮氨酸选择性抑制。对PLAP和GCAP中12个不同残基中的5个进行定点诱变，结果表明，Gly429是L-亮氨酸抑制GCAP的主要决定因素，Ser84和Leu297在抑制过程中起调节作用。
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