共聚焦显微镜概述。

Overview of confocal microscopy.

作者信息

Swaim William D

机构信息

Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2010;588:187-201. doi: 10.1007/978-1-59745-324-0_21.

DOI:10.1007/978-1-59745-324-0_21

PMID:20012832

Abstract

Born out of the need to overcome an imaging problem in the 1950s, confocal microscopes today allow researchers to go beyond simple imaging and ask biochemical questions. This chapter provides background information on the development of modern confocal microscopes, their uses and applications. Sample preparation and observation are also discussed. Information is also provided about more advanced applications such as FRAP, FRET and 2-photon imaging. The requirements for setting up a confocal laboratory and the instrumentation needs are also discussed.

摘要

共聚焦显微镜诞生于20世纪50年代克服成像问题的需求，如今它使研究人员能够超越简单成像，进而探讨生物化学问题。本章提供了有关现代共聚焦显微镜的发展、用途及应用的背景信息。还讨论了样品制备和观察。此外，还介绍了诸如光漂白荧光恢复（FRAP）、荧光共振能量转移（FRET）和双光子成像等更先进的应用。同时也讨论了建立共聚焦实验室的要求和仪器需求。