Oliver Constance
Department of Cell and Molecular Biology, Faculdade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Methods Mol Biol. 2010;588:311-6. doi: 10.1007/978-1-59745-324-0_30.
Although gold particles are readily detectable by transmission electron microscopy, they can be difficult to visualize by bright-field light microscopy. However when the gold is silver-enhanced it is easy to see. During silver-enhancement, the colloidal gold serves as a nucleation site for the deposition of metallic silver. The enhancing solutions are physical developers that contain both silver ions and a reducing agent, buffered to an acid pH. The silver-enhancement method has also been used successfully to enlarge small-diameter gold particles for visualization by scanning electron microscopy. Silver-enhancement has been applied to a wide variety of tissues and antigens for both light and scanning electron microscopy.
虽然通过透射电子显微镜很容易检测到金颗粒，但用明场光学显微镜却很难观察到它们。然而，当金进行银增强处理后就很容易看到了。在银增强过程中，胶体金作为金属银沉积的成核位点。增强溶液是物理显影剂，含有银离子和还原剂，缓冲至酸性pH值。银增强方法也已成功用于扩大小直径金颗粒，以便通过扫描电子显微镜进行观察。银增强已应用于多种组织和抗原，用于光学显微镜和扫描电子显微镜观察。
