通过免疫细胞化学、扫描和透射电子显微镜以及蛋白质印迹法检测精子膜蛋白

Detection of Sperm Membrane Proteins by Immunocytochemistry, Scanning and Transmission Electron Microscopy, and Western Blotting.

作者信息

Pinart Elisabeth

机构信息

Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.

Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, Girona, Spain.

出版信息

Methods Mol Biol. 2025;2897:445-455. doi: 10.1007/978-1-0716-4406-5_29.

DOI:10.1007/978-1-0716-4406-5_29

PMID:40202652

Abstract

Western blotting in combination with scanning and transmission electron microscopy provide accurate and complementary information on the presence and localization of proteins in the plasma membrane, as well as the structural and ultrastructural features of sperm cells. The present chapter describes the procedures to detect the presence of membrane proteins by Western blotting and to localize them in the plasma membrane of sperm cells.

摘要

蛋白质免疫印迹法结合扫描电子显微镜和透射电子显微镜，能够提供关于质膜中蛋白质的存在和定位以及精子细胞的结构和超微结构特征的准确且互补的信息。本章描述了通过蛋白质免疫印迹法检测膜蛋白的存在并将其定位在精子细胞质膜中的方法。