Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA.
Mol Imaging Biol. 2010 Jun;12(3):325-34. doi: 10.1007/s11307-009-0274-4. Epub 2009 Dec 15.
We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium.
Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI.
Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals.
Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.
我们通过检测假设,即微环境的调节(使用抗氧化剂)将增加干细胞在缺氧和移植到心肌后的存活率。
利用生物发光成像(BLI),稳定转染报告基因(萤火虫荧光素酶)的大鼠心肌细胞。首先,我们研究了在缺氧条件下细胞中氧化应激的作用。随后,使用高分辨率超声将干细胞移植到大鼠的心肌中,并使用 BLI 每天监测其存活情况。
与对照细胞相比,在缺氧条件下,氧化应激增加,细胞存活率降低,而抗氧化剂可以保存这两种情况。在活体中,与未处理的细胞/动物相比,移植到心肌后氧化应激阻断增加了早期细胞存活率。
局部微环境的调节(使用抗氧化剂)可提高干细胞的存活率。增加对干细胞及其微环境之间相互作用的理解,对于推进再生医学领域至关重要。