抗氧化剂可提高心肌内移植的心肌细胞的早期存活率。
Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium.
机构信息
Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA.
出版信息
Mol Imaging Biol. 2010 Jun;12(3):325-34. doi: 10.1007/s11307-009-0274-4. Epub 2009 Dec 15.
Abstract
PURPOSE
We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium.
PROCEDURES
Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI.
RESULTS
Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals.
CONCLUSION
Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.
摘要
目的
我们通过检测假设，即微环境的调节（使用抗氧化剂）将增加干细胞在缺氧和移植到心肌后的存活率。
方法
利用生物发光成像（BLI），稳定转染报告基因（萤火虫荧光素酶）的大鼠心肌细胞。首先，我们研究了在缺氧条件下细胞中氧化应激的作用。随后，使用高分辨率超声将干细胞移植到大鼠的心肌中，并使用 BLI 每天监测其存活情况。
结果
与对照细胞相比，在缺氧条件下，氧化应激增加，细胞存活率降低，而抗氧化剂可以保存这两种情况。在活体中，与未处理的细胞/动物相比，移植到心肌后氧化应激阻断增加了早期细胞存活率。
结论
局部微环境的调节（使用抗氧化剂）可提高干细胞的存活率。增加对干细胞及其微环境之间相互作用的理解，对于推进再生医学领域至关重要。
相似文献
1
Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium.抗氧化剂可提高心肌内移植的心肌细胞的早期存活率。
Mol Imaging Biol. 2010 Jun;12(3):325-34. doi: 10.1007/s11307-009-0274-4. Epub 2009 Dec 15.
2
Role of oxidative stress in hypoxia preconditioning of cells transplanted to the myocardium: a molecular imaging study.氧化应激在移植到心肌的细胞缺氧预处理中的作用：一项分子成像研究。
J Cardiovasc Surg (Torino). 2011 Aug;52(4):579-85.
3
Collagen matrices enhance survival of transplanted cardiomyoblasts and contribute to functional improvement of ischemic rat hearts.胶原蛋白基质可提高移植心肌母细胞的存活率，并有助于改善缺血大鼠心脏的功能。
Circulation. 2006 Jul 4;114(1 Suppl):I167-73. doi: 10.1161/CIRCULATIONAHA.105.001297.
4
Noninvasive monitoring of oxidative stress in transplanted mesenchymal stromal cells.移植间充质基质细胞氧化应激的无创监测。
JACC Cardiovasc Imaging. 2013 Jul;6(7):795-802. doi: 10.1016/j.jcmg.2012.11.018. Epub 2013 May 1.
5
In vivo bioluminescence imaging of cord blood derived mesenchymal stem cell transplantation into rat myocardium.脐血来源的间充质干细胞移植到大鼠心肌的体内生物发光成像。
Ann Nucl Med. 2006 Apr;20(3):165-70. doi: 10.1007/BF03027425.
6
Embryonic cardiomyocyte, but not autologous stem cell transplantation, restricts infarct expansion, enhances ventricular function, and improves long-term survival.胚胎心肌细胞，但不是自体干细胞移植，可以限制梗死扩张，增强心室功能，并提高长期存活率。
PLoS One. 2013 Apr 9;8(4):e61510. doi: 10.1371/journal.pone.0061510. Print 2013.
7
Noninvasive evaluation of immunosuppressive drug efficacy on acute donor cell survival.免疫抑制药物对急性供体细胞存活疗效的无创评估
Mol Imaging Biol. 2006 May-Jun;8(3):163-70. doi: 10.1007/s11307-006-0038-3.
8
Dalspinin isolated from Spermacoce hispida (Linn.) protects H9c2 cardiomyocytes from hypoxic injury by modulating oxidative stress and apoptosis.从水蓑衣（Linn.）中分离出的 Dalspinin 通过调节氧化应激和细胞凋亡来保护 H9c2 心肌细胞免受缺氧损伤。
J Ethnopharmacol. 2019 Sep 15;241:111962. doi: 10.1016/j.jep.2019.111962. Epub 2019 May 21.
9
In vivo assessment of the electrophysiological integration and arrhythmogenic risk of myocardial cell transplantation strategies.在体评估心肌细胞移植策略的电生理整合和致心律失常风险。
Stem Cells. 2010 Dec;28(12):2151-61. doi: 10.1002/stem.545.
10
Effects of epigenetic modulation on reporter gene expression: implications for stem cell imaging.表观遗传调控对报告基因表达的影响：对干细胞成像的意义
FASEB J. 2006 Jan;20(1):106-8. doi: 10.1096/fj.05-4551fje. Epub 2005 Oct 24.
引用本文的文献
1
Identification of a Sesquiterpene Lactone from Leaves with Antioxidant Activity in Primary Human Muscle Cells.从叶片中鉴定出一种具有抗氧化活性的倍半萜内酯。
Molecules. 2021 Mar 2;26(5):1328. doi: 10.3390/molecules26051328.
2
Pathway-specific reporter genes to study stem cell biology.用于研究干细胞生物学的途径特异性报告基因。
Stem Cells. 2020 Jun;38(6):808-814. doi: 10.1002/stem.3167. Epub 2020 Mar 28.
3
Ginkgo Biloba L. Extract Reduces H2O2-Induced Bone Marrow Mesenchymal Stem Cells Cytotoxicity by Regulating Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways and Oxidative Stress.银杏叶提取物通过调节丝裂原活化蛋白激酶（MAPK）信号通路和氧化应激减轻 H2O2 诱导的骨髓间充质干细胞的细胞毒性。
Med Sci Monit. 2018 May 14;24:3159-3167. doi: 10.12659/MSM.910718.
4
The cardiac regenerative potential of myoblasts remains limited despite improving their survival via antioxidant treatment.尽管通过抗氧化剂治疗提高了成肌细胞的存活率，但其心脏再生潜力仍然有限。
CellR4 Repair Replace Regen Reprogram. 2014;2(2). Epub 2014 Mar 31.
5
Image-guided stem cells with functionalized self-assembling peptide nanofibers for treatment of acute myocardial infarction in a mouse model.用于小鼠急性心肌梗死治疗的图像引导下的具有功能化自组装肽纳米纤维的干细胞
Am J Transl Res. 2017 Aug 15;9(8):3723-3731. eCollection 2017.
6
Effects of Antioxidant Supplements on the Survival and Differentiation of Stem Cells.抗氧化剂补充剂对干细胞存活和分化的影响。
Oxid Med Cell Longev. 2017;2017:5032102. doi: 10.1155/2017/5032102. Epub 2017 Jul 9.
7
Sitagliptin ameliorates oxidative stress in experimental diabetic nephropathy by diminishing the miR-200a/Keap-1/Nrf2 antioxidant pathway.西他列汀通过减弱miR-200a/Keap-1/Nrf2抗氧化途径改善实验性糖尿病肾病中的氧化应激。
Diabetes Metab Syndr Obes. 2017 Jun 7;10:207-222. doi: 10.2147/DMSO.S132537. eCollection 2017.
8
New strategy to rescue the inhibition of osteogenesis of human bone marrow-derived mesenchymal stem cells under oxidative stress: combination of vitamin C and graphene foams.挽救氧化应激下人骨髓间充质干细胞成骨抑制的新策略：维生素C与石墨烯泡沫的联合应用
Oncotarget. 2016 Nov 1;7(44):71998-72010. doi: 10.18632/oncotarget.12456.
9
Molecular imaging of stem cells for the treatment of acute myocardial infarction.用于急性心肌梗死治疗的干细胞分子成像
Int J Clin Exp Med. 2015 Jun 15;8(6):8938-47. eCollection 2015.
10
Mesenchymal Stromal Cells Improve Renovascular Function in Polycystic Kidney Disease.间充质基质细胞改善多囊肾病中的肾血管功能。
Cell Transplant. 2015;24(9):1687-98. doi: 10.3727/096368914X684619. Epub 2014 Oct 6.
本文引用的文献
1
Essential role of NOXA1 in generation of reactive oxygen species induced by oxidized low-density lipoprotein in human vascular endothelial cells.NOXA1在氧化型低密度脂蛋白诱导人血管内皮细胞产生活性氧物种中的重要作用。
Endothelium. 2008 May-Jun;15(3):137-41. doi: 10.1080/10623320802125433.
2
Production of reactive oxygen species by complex I (NADH:ubiquinone oxidoreductase) from Escherichia coli and comparison to the enzyme from mitochondria.大肠杆菌中复合物I（NADH：泛醌氧化还原酶）产生活性氧物种及其与线粒体中该酶的比较。
Biochemistry. 2008 Mar 25;47(12):3964-71. doi: 10.1021/bi702243b. Epub 2008 Feb 29.
3
Reactive oxygen species and p47phox activation are essential for the Mycobacterium tuberculosis-induced pro-inflammatory response in murine microglia.活性氧物种和p47phox激活对于结核分枝杆菌诱导的小鼠小胶质细胞促炎反应至关重要。
J Neuroinflammation. 2007 Nov 26;4:27. doi: 10.1186/1742-2094-4-27.
4
Chronic proteasome inhibition contributes to coronary atherosclerosis.慢性蛋白酶体抑制作用会导致冠状动脉粥样硬化。
Circ Res. 2007 Oct 26;101(9):865-74. doi: 10.1161/CIRCRESAHA.107.152959. Epub 2007 Sep 6.
5
Hydrogen peroxide mediates a transient vasorelaxation with tempol during oxidative stress.在氧化应激期间，过氧化氢与Tempol共同介导短暂的血管舒张。
Am J Physiol Heart Circ Physiol. 2007 Oct;293(4):H2085-92. doi: 10.1152/ajpheart.00968.2006. Epub 2007 Jul 20.
6
Pharmacologically preconditioned skeletal myoblasts are resistant to oxidative stress and promote angiomyogenesis via release of paracrine factors in the infarcted heart.经药理学预处理的骨骼肌成肌细胞对氧化应激具有抗性，并通过在梗死心脏中释放旁分泌因子促进血管生成。
Circ Res. 2007 Mar 2;100(4):545-55. doi: 10.1161/01.RES.0000258460.41160.ef. Epub 2007 Jan 18.
7
Stem cell therapy after myocardial infarction: ready for clinical application?心肌梗死后的干细胞治疗：准备好用于临床应用了吗？
Curr Opin Mol Ther. 2006 Oct;8(5):396-414.
8
Adenoviral human BCL-2 transgene expression attenuates early donor cell death after cardiomyoblast transplantation into ischemic rat hearts.腺病毒介导的人BCL-2转基因表达可减轻心肌母细胞移植到缺血大鼠心脏后早期供体细胞的死亡。
Circulation. 2006 Jul 4;114(1 Suppl):I174-80. doi: 10.1161/CIRCULATIONAHA.105.001370.
9
In vivo imaging using bioluminescence: a tool for probing graft-versus-host disease.使用生物发光的体内成像：一种探究移植物抗宿主病的工具。
Nat Rev Immunol. 2006 Jun;6(6):484-90. doi: 10.1038/nri1879.
10
Enhanced cell transplantation: preventing apoptosis increases cell survival and ventricular function.增强型细胞移植：预防细胞凋亡可提高细胞存活率和心室功能。
Am J Physiol Heart Circ Physiol. 2006 Aug;291(2):H939-47. doi: 10.1152/ajpheart.00155.2006. Epub 2006 Mar 31.
Zotero 插件