Leibniz Institute for Polymer Research, Max Bergmann Centre for Biomaterials, Dresden, Germany.
J Tissue Eng Regen Med. 2010 Jun;4(4):324-7. doi: 10.1002/term.240.
The critical requirement for matrix-associated bone morphogenetic proteins (BMPs) during induction of bone formation in vivo has long been recognized. However, the role of extracellular matrix (ECM) physisorbed BMPs in inducing the differentiation of resident mesenchymal stem cells into osteoblasts has been ill-defined. We therefore used BMP-responsive C2C12s to study the biological activity of collagen type I physisorbed BMP-2. Fibrillar collagen type I scaffolds were loaded with 75 ng BMP-2/microg collagen. Under cell culture conditions, 40% of loaded (125)I-labelled BMP-2 was released within 24 h, whereas the remaining BMP-2 was stably physisorbed for > 7 days. Using these systems suggested that physisorbed BMP-2 is more active than diffusible BMP-2. Thus, the current clinical practice of immobilizing BMPs on collagen type I scaffolds not only prolongs local delivery of the morphogen but could also enhance biological activity at the cellular level.
长期以来，人们一直认识到细胞外基质（ECM）吸附的骨形态发生蛋白（BMPs）在诱导体内骨形成中的关键要求。然而，细胞外基质吸附的 BMP 在诱导驻留间充质干细胞分化为成骨细胞中的作用一直没有明确界定。因此，我们使用 BMP 反应性 C2C12 来研究 I 型胶原吸附的 BMP-2 的生物学活性。纤维状 I 型胶原支架负载 75ng BMP-2/μg 胶原。在细胞培养条件下，24 小时内释放了 40%负载的（125）I 标记的 BMP-2，而其余的 BMP-2 稳定地吸附超过 7 天。使用这些系统表明，吸附的 BMP-2 比弥散的 BMP-2 更具活性。因此，目前将 BMP 固定在 I 型胶原支架上的临床实践不仅可以延长形态发生素的局部递送，而且还可以增强细胞水平的生物学活性。
