鼻咽癌中针对过氧化氢酶和超氧化物歧化酶的自身抗体水平较高。

High levels of autoantibodies against catalase and superoxide dismutase in nasopharyngeal carcinoma.

作者信息

Gargouri Bochra, Lassoued Saloua, Ben Mansour Riadh, Ayadi Wajdi, Idriss Nabil, Attia Hammadi, El Feki Abd El Fatteh

机构信息

Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, Tunisia.

出版信息

South Med J. 2009 Dec;102(12):1222-6. doi: 10.1097/SMJ.0b013e3181bb85b5.

DOI:10.1097/SMJ.0b013e3181bb85b5

PMID:20016428

Abstract

INTRODUCTION

Autoantibodies against the antioxidant enzymes have been described in Epstein-Barr virus-associated diseases. Here, we hypothesize that Epstein-Barr virus (EBV), which is associated with nasopharyngeal carcinoma (NPC), induces anticatalase and/or antisuperoxide dismutase autoantibodies that inhibit catalase and/or superoxide dismutase activities and thereby contribute to the oxidative stress status described in this pathology.

METHODS

Using a standard enzyme-linked immunosorbent assay (ELISA), the levels of immunoglobulin G (IgG), and M (IgM) directed against catalase and superoxide dismutase in the sera of 30 NPC patients and 30 healthy control individuals were evaluated. The antioxidative profile was tested among the same patients by measuring serum catalase and superoxide dismutase activities. To investigate the implication of EBV in the establishment of autoantibody production in NPC patients, a correlation study between serological testing for EBV viral capsid antigen immunoglobulin G (VCA IgG) and autoantibodies against both enzymes was undertaken.

FINDINGS

The levels of IgG against superoxide dismutase and catalase were found to be increased in sera patients compared to controls (P<0.001). NPC patients exhibited decreased catalase (P<0.001) and superoxide dismutase activities (P<0.001) in their sera. However, a positive correlation between superoxide dismutase IgM antibody and IgG antibody titers to VCA (P<0.05, r=0.483, n=21) was found. A positive correlation between catalase (IgM) antibodies and IgG antibody titers to VCA (P<0.05, r=0.546, n=30) was also found.

CONCLUSION

High levels of anticatalase and antisuperoxide dismutase antibodies in the sera of NPC patients were found.

摘要

引言

在爱泼斯坦-巴尔病毒相关疾病中已发现针对抗氧化酶的自身抗体。在此，我们假设与鼻咽癌（NPC）相关的爱泼斯坦-巴尔病毒（EBV）会诱导抗过氧化氢酶和/或抗超氧化物歧化酶自身抗体，这些抗体抑制过氧化氢酶和/或超氧化物歧化酶活性，从而导致该病理状态下所描述的氧化应激状态。

方法

采用标准酶联免疫吸附测定（ELISA）评估30例NPC患者和30例健康对照个体血清中针对过氧化氢酶和超氧化物歧化酶的免疫球蛋白G（IgG）和M（IgM）水平。通过测量血清过氧化氢酶和超氧化物歧化酶活性，对相同患者的抗氧化谱进行检测。为研究EBV在NPC患者自身抗体产生过程中的作用，对EBV病毒衣壳抗原免疫球蛋白G（VCA IgG）血清学检测与针对这两种酶的自身抗体进行相关性研究。

结果

与对照组相比，患者血清中抗超氧化物歧化酶和过氧化氢酶的IgG水平升高（P<0.001）。NPC患者血清中的过氧化氢酶（P<0.001）和超氧化物歧化酶活性（P<0.001）降低。然而，发现超氧化物歧化酶IgM抗体与VCA的IgG抗体滴度呈正相关（P<0.05，r=0.483，n=21）。还发现过氧化氢酶（IgM）抗体与VCA的IgG抗体滴度呈正相关（P<0.05，r=0.546，n=30）。