Shimakage M, Ikegami N, Chatani M, Yoshino K, Sato T
Clinical Research Institute, Osaka National Hospital, Japan.
Biken J. 1987 Jun;30(2):45-51.
Serological follow-up studies for up to 4 years on the levels of IgG antibody to EBV-determined nuclear antigen (EBNA) were carried out on 36 patients with nasopharyngeal carcinoma (NPC). The serum levels of IgA antibody specific to EBV capsid antigen (VCA) were also measured in some of the patients. The titers of EBNA antibody were measured by enzyme-linked immunosorbent assay (ELISA) and those of IgA antibody to VCA were measured by the indirect immunofluorescence method. The EBNA antibody titers in most sera from the patients before radiation therapy were found to be at least 4 times the mean values in the sera of healthy control adults. Within 2 to 8 months after completion of therapy by 4-MV liniac X-ray irradiation with total doses of 60 to 80 Gy, the titers of EBNA antibody in the sera of 6 patients had returned to normal levels, and low levels of EBNA antibody were maintained for a long time after therapy. These serological data were associated with a good clinical prognosis without recurrence or metastases. But in 6 patients, the patterns of change in the EBNA antibody levels were different: the levels remained high after therapy or first decreased to the normal level and then rose to at least 4 times this level. These 6 patients showed recurrence or metastases. The patterns of change in the EBNA antibody levels were well correlated with those of change in the levels of IgA antibody specific to VCA.(ABSTRACT TRUNCATED AT 250 WORDS)
对36例鼻咽癌患者进行了长达4年的血清学随访研究，检测其针对EB病毒核抗原（EBNA）的IgG抗体水平。部分患者还检测了针对EB病毒衣壳抗原（VCA）的IgA抗体血清水平。采用酶联免疫吸附测定（ELISA）法检测EBNA抗体滴度，采用间接免疫荧光法检测针对VCA的IgA抗体滴度。发现大多数患者放疗前血清中的EBNA抗体滴度至少是健康对照成年人血清平均值的4倍。在用4兆伏直线加速器X射线进行总剂量60至80戈瑞的放疗结束后2至8个月内，6例患者血清中的EBNA抗体滴度恢复到正常水平，且治疗后很长一段时间内维持在低水平。这些血清学数据与良好的临床预后相关，无复发或转移。但有6例患者的EBNA抗体水平变化模式不同：治疗后水平仍高，或先降至正常水平，然后升至至少此水平的4倍。这6例患者出现了复发或转移。EBNA抗体水平的变化模式与针对VCA的IgA抗体水平的变化模式密切相关。（摘要截短于250字）
