Pardo Lorena, Machado Virginia, Mollerach Marta, Mota María Inés, Tuchscherr Lorena P N, Gadea Pilar, Gardella Noella, Sordelli Daniel O, Vola Magdalena, Schelotto Felipe, Varela Gustavo
Department of Bacteriology and Virology, Institute of Hygiene, School of Medicine, Universidad de la República, Alfredo Navarro, 3051 Montevideo, Uruguay.
Int J Microbiol. 2009;2009:472126. doi: 10.1155/2009/472126. Epub 2009 Oct 22.
We analyzed 90 nonduplicates community-associated methicillin-resistant S. aureus (CA-MRSA) strains isolated from skin and soft-tissue infections. All strains were mecA positive. Twenty-four of the 90 strains showed inducible macrolide-lincosamide-streptogramin B resistance. All strains produced alpha-toxin; 96% and 100% of them displayed positive results for lukS-F and cna genes, respectively. Eigthy-five strains expressed capsular polysaccharide serotype 8. Six different pulsotypes were discriminated by pulsed-field gel electrophoresis (PFGE) and three predominant groups of CA-MRSA strains (1, 2, and 4) were identified, in agreement with phenotypic and genotypic characteristics. Strains of group 1 (pulsotype A, CP8+, and Panton-Valentine leukocidin (PVL)+) were the most frequently recovered and exhibited a PFGE band pattern identical to other CA-MRSA strains previously isolated in Uruguay and Brazil. Three years after the first local CA-MRSA report, these strains are still producing skin and soft-tissue infections demonstrating the stability over time of this community-associated emerging pathogen.
我们分析了从皮肤和软组织感染中分离出的90株非重复的社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）菌株。所有菌株mecA均呈阳性。90株菌株中有24株表现出诱导性大环内酯-林可酰胺-链阳菌素B耐药。所有菌株均产生α毒素；其中96%和100%的菌株lukS-F和cna基因检测分别呈阳性。85株菌株表达荚膜多糖血清型8。通过脉冲场凝胶电泳（PFGE）区分出六种不同的脉冲型，并鉴定出三组主要的CA-MRSA菌株（1、2和4），这与表型和基因型特征一致。第1组菌株（脉冲型A、CP8+和杀白细胞素（PVL）+）是最常分离到的，其PFGE条带模式与之前在乌拉圭和巴西分离出的其他CA-MRSA菌株相同。在首次本地报告CA-MRSA三年后，这些菌株仍在引起皮肤和软组织感染，表明这种社区获得性新兴病原体随时间推移具有稳定性。
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