Özekinci Tuncer, Dal Tuba, Yanık Keramettin, Özcan Nida, Can Şükran, Tekin Alicem, Yıldırım Halil İbrahim, Kandemir İdris
Department of Medical Microbiology, Faculty of Medicine, Dicle University , Diyarbakır , Turkey.
Department of Medical Microbiology, Faculty of Medicine, Yıldırım Beyazıt University , Ankara , Turkey.
Biotechnol Biotechnol Equip. 2014 Nov 2;28(6):1089-1094. doi: 10.1080/13102818.2014.976457. Epub 2014 Nov 7.
causes serious hospital-acquired (HA) and community-acquired (CA) infections. Skin and soft-tissue infections especially are sometimes caused by strains harbouring Panton-Valentine leukocidin (PVL). PVL belongs to a family of bi-component leukocidal toxins produced by staphylococci. It is a pore-forming toxin encoded by and . A total of 70 strains: 38 (54%) methicillin-resistant (MRSA) and 32 (46%) methicillin-susceptible (MSSA), were isolated from patients admitted to Dicle University Hospital (Turkey). Identification of and antibiotics-susceptibility testing were performed with PHOENIX 100. PVL genes and genes were detected by polymerase chain reaction. Of the 70 studied strains, 36 ones (51%) were community acquired and 34 ones (49%) were hospital acquired . A total of 38 (54%) strains were positive for (), of which 32 ones (84%) were HA. Of the strains, 30 (94%) were CA. Of the 70 studied strains, 12 (17%) strains were PVL: 8 (22%) of the 36 CA strains and 4 (12%) of the 34 HA strains. Of the 12 PVL strains, 4 strains were . The PVL positivity rate was 25% in MSSA, whereas 10.5% in MRSA. Of the overall PVL strains, seven strains were obtained from wounds; four ones from skin abscess; and one from blood culture. Taken together, the obtained results showed a substantial level of PVL genes in the studied region. Although PVL is known as a common virulence factor of CA MRSA, HA MRSA isolates in our study showed a considerable rate of PVL positivity.
会引发严重的医院获得性（HA）感染和社区获得性（CA）感染。皮肤和软组织感染有时尤其由携带杀白细胞素（PVL）的菌株引起。PVL属于葡萄球菌产生的双组分杀白细胞毒素家族。它是一种由……和……编码的成孔毒素。从土耳其迪克莱大学医院收治的患者中分离出70株菌株：38株（54%）耐甲氧西林（MRSA）和32株（46%）对甲氧西林敏感（MSSA）。使用PHOENIX 100进行……鉴定和抗生素敏感性测试。通过聚合酶链反应检测PVL基因和……基因。在所研究的70株菌株中，36株（51%）是社区获得性的，34株（49%）是医院获得性的。共有38株（54%）菌株……呈阳性（……），其中32株（84%）是医院获得性的。在……菌株中，30株（94%）是社区获得性的。在所研究的70株菌株中，12株（17%）菌株携带PVL：36株社区获得性菌株中的8株（22%）和34株医院获得性菌株中的4株（12%）。在12株携带PVL的菌株中，4株是……。MSSA中的PVL阳性率为25%，而MRSA中的为10.5%。在所有携带PVL的菌株中，7株来自伤口；4株来自皮肤脓肿；1株来自血培养。总体而言，所获结果表明在所研究地区PVL基因水平较高。尽管PVL是社区获得性MRSA的常见毒力因子，但我们研究中的医院获得性MRSA分离株显示出相当高的PVL阳性率。
