Istituto di Biofisica, CNR, Pisa, Italy.
Cell Mol Life Sci. 2010 Mar;67(6):965-71. doi: 10.1007/s00018-009-0225-x. Epub 2009 Dec 18.
Here, we report the DNA sequence of the rhodopsin gene in the alga Cyanophora paradoxa (Glaucophyta). The primers were designed according to the conserved regions of prokaryotic and eukaryotic rhodopsin-like proteins deposited in the GenBank. The sequence consists of 1,272 bp comprised of 5 introns. The correspondent protein, named Cyanophopsin, showed high identity to rhodopsin-like proteins of Archea, Bacteria, Fungi, and Algae. At the N-terminal, the protein is characterized by a region with no transmembrane alpha-helices (80 aa), followed by a region with 7alpha-helices (219 aa) and a shorter 35-aa C-terminal region. The DNA sequence of the N-terminal region was expressed in E. coli and the recombinant purified peptide was used as antigen in hens to obtain polyclonal antibodies. Indirect immunofluorescence in C. paradoxa cells showed a marked labeling of the muroplast (aka cyanelle) membrane.
在这里,我们报告了蓝藻(Glaucophyta)中的视蛋白基因的 DNA 序列。根据 GenBank 中已存入的原核和真核类视蛋白的保守区域设计了引物。该序列由 5 个内含子组成,共 1272bp。对应的蛋白,命名为 Cyanophopsin,与古菌、细菌、真菌和藻类的类视蛋白具有高度的相似性。在 N 端,该蛋白的特征是一个没有跨膜α螺旋的区域(80 个氨基酸),其后是一个具有 7 个α螺旋的区域(219 个氨基酸)和一个较短的 35 个氨基酸的 C 端区域。N 端区域的 DNA 序列在大肠杆菌中进行了表达,纯化后的重组肽被用作母鸡的抗原以获得多克隆抗体。在 C. paradoxa 细胞中的间接免疫荧光显示,质膜(也称为蓝藻体)有明显的标记。