钙结合蛋白调钙素对大鼠肝细胞核中Ca2(+)-激活的DNA片段化的抑制作用。
Inhibitory effect of calcium-binding protein regucalcin on Ca2(+)-activated DNA fragmentation in rat liver nuclei.
作者信息
Yamaguchi M, Sakurai T
机构信息
Department of Environmental Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
出版信息
FEBS Lett. 1991 Feb 25;279(2):281-4. doi: 10.1016/0014-5793(91)80168-3.
Incubation of isolated rat liver nuclei with ATP, NAD+, and micromolar Ca2+ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 1.0 microM Ca2+ added, and saturation of the process was observed with 10 microM Ca2+. The Ca2+ (10 microM)-activated DNA fragmentation was inhibited by the presence of Ca2(+)-binding protein regucalcin isolated from rat liver cytosol. The inhibitory effect of regucalcin was complete at 0.5 microM. At 25 microM Ca2+ added, such an effect of regucalcin (1.0 microM) was not seen. Regucalcin also inhibited Ca2(+)-activated DNA fragmentation in the presence of calmodulin (10 and 20 micrograms). The results show that regucalcin can inhibit the Ca2(+)-activated DNA fragmentation due to binding the metal, suggesting a role in regulation of liver nuclear functions.
将分离的大鼠肝细胞核与ATP、NAD⁺以及微摩尔浓度的各种金属离子（钙离子）一同孵育，会导致广泛的DNA水解。添加1.0微摩尔钙离子时出现半数最大活性，而在添加10微摩尔钙离子时观察到该过程达到饱和。从大鼠肝细胞溶质中分离出的钙离子结合蛋白调钙素的存在会抑制10微摩尔钙离子激活的DNA片段化。调钙素在0.5微摩尔时具有完全抑制作用。添加25微摩尔钙离子时，未观察到1.0微摩尔调钙素的这种作用。在存在钙调蛋白（10微克和20微克）的情况下，调钙素也会抑制钙离子激活的DNA片段化。结果表明，调钙素可通过结合金属来抑制钙离子激活的DNA片段化，提示其在肝细胞核功能调节中发挥作用。
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