Department of Crop and Soil Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
J Agric Food Chem. 2010 Jan 13;58(1):218-25. doi: 10.1021/jf9038106.
The objective of this research was to identify and quantify the phenolic and short-chained aliphatic organic acids present in the seeds of three wild-type populations of wild oat and compare these results to the chemical composition of seeds from two commonly utilized wild oat isolines (M73 and SH430). Phenolic acids have been shown to serve as germination inhibitors, as well as protection for seeds from biotic and abiotic stress factors in other species, whereas aliphatic organic acids have been linked to germination traits and protection against pathogens. Wild oat populations were grown under a "common garden" environment to remove maternal variation, and the resulting seeds were extracted to remove the readily soluble and chemically bound phenolic and aliphatic organic acid components. Compounds were identified and quantified using gas chromatography-mass spectrometry. Ferulic and p-coumaric acid comprised 99% of the total phenolic acids present in the seeds, of which 91% were contained in the hulls and 98% were in the chemically bound forms. Smaller quantities of OH benzoic and vanillic acid were also detected. Soluble organic acids concentrations were higher in the M73 isoline compared to SH430, suggesting that these chemical constituents could be related to seed dormancy. Malic, succinic, fumaric and azelaic acid were the dominant aliphatic organic acids detected in all seed and chemical fractions.
本研究的目的是鉴定和量化三种野生燕麦种群种子中存在的酚类和短链脂肪族有机酸，并将这些结果与两种常用野生燕麦分离株（M73 和 SH430）种子的化学成分进行比较。已经表明，在其他物种中，酚酸可以作为发芽抑制剂，并保护种子免受生物和非生物胁迫因素的影响，而脂肪族有机酸与发芽特性和抵御病原体有关。野生燕麦种群在“共同花园”环境中生长，以去除母体变异，然后提取得到的种子以去除易溶的和化学结合的酚类和脂肪族有机酸成分。使用气相色谱-质谱法鉴定和定量化合物。阿魏酸和对香豆酸构成了种子中存在的总酚酸的 99%，其中 91%存在于种皮中，98%以化学结合的形式存在。还检测到少量的 OH 苯甲酸和香草酸。与 SH430 相比，M73 分离株中的可溶性有机酸浓度更高，这表明这些化学物质可能与种子休眠有关。在所有种子和化学部分中，检测到的主要脂肪族有机酸是苹果酸、琥珀酸、富马酸和壬二酸。
