Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom.
ACS Chem Biol. 2009 Dec 18;4(12):983-5. doi: 10.1021/cb900304p.
An improved method for the semisynthesis of a potassium channel involving native chemical ligation allows the introduction of short sequences containing non-canonical amino acids at any position within the polypeptide chain. The work enhances the technology available for a range of fundamental investigations of membrane proteins and for applications of membrane channels and pores in biotechnology.
一种改进的半合成钾通道的方法，涉及到天然化学连接，允许在多肽链的任何位置引入含有非典型氨基酸的短序列。这项工作增强了可用于一系列膜蛋白基础研究以及膜通道和孔在生物技术中的应用的技术。
