Department and Graduate Program of Bioindustry Technology, Dayeh University, Chang-Hua, 515, Taiwan.
N Biotechnol. 2010 Feb 28;27(1):89-93. doi: 10.1016/j.nbt.2009.12.002. Epub 2009 Dec 16.
In this study, optimization of enzymatic synthesis of caffeic acid phenethyl ester (CAPE), catalyzed by immobilized lipase (Novozym 435) from Candida antarctica was investigated. Novozym 435 was used to catalyze caffeic acid and 2-phenylethanol in an isooctane system. Response surface methodology (RSM) and 5-level-4-factor central-composite rotatable design (CCRD) were employed to evaluate the effects of synthesis parameters, such as reaction temperature (30-70 degrees C), reaction time (24-72 hours), substrate molar ratio of caffeic acid to 2-phenylethanol (1:10-1:90) and enzyme amounts (100-500 PLU) on percentage conversion of CAPE by direct esterification. Reaction temperature and time had significant effects on percent conversion. On the basis of ridge max analysis, the optimum conditions for synthesis were: reaction time 59 hours, reaction temperature 69 degrees C, substrate molar ratio 1:72 and enzyme amount 351 PLU. The molar conversion of predicted values and actual experimental values were 91.86+/-5.35% and 91.65+/-0.66%, respectively.
本研究考察了固定化脂肪酶（南极假丝酵母脂肪酶 Novozym 435）催化合成咖啡酸苯乙酯（CAPE）的条件优化。Novozym 435 用于催化没食子酸和 2-苯乙醇在异辛烷体系中的酯化反应。采用响应面法（RSM）和 5 水平 4 因素中心组合旋转设计（CCRD）考察了反应温度（30-70℃）、反应时间（24-72 小时）、咖啡酸与 2-苯乙醇摩尔比（1:10-1:90）和酶用量（100-500PLU）等合成参数对直接酯化法合成 CAPE 转化率的影响。反应温度和时间对转化率有显著影响。基于岭脊最大值分析，合成的最佳条件为：反应时间 59 小时，反应温度 69℃，底物摩尔比 1:72，酶用量 351PLU。预测值和实际实验值的摩尔转化率分别为 91.86+/-5.35%和 91.65+/-0.66%。
