Sachedina S, Greiner J V, Glonek T
MR Laboratory, Chicago College of Osteopathic Medicine, IL 60615.
Invest Ophthalmol Vis Sci. 1991 Mar;32(3):625-32.
The authors compared porcine corneal and scleral membrane phospholipids determined with use of 31P nuclear magnetic resonance (NMR). These tissues make up the tunica fibrosa (outer coat) of the eye. Since the sclera, unlike the cornea, does not possess an epithelium or an endothelium, comparative analysis of these tissues included examination of the cornea with and without its epithelium and endothelium. The phospholipids quantified include: phosphatidylcholine, lysophosphatidylcholine, phosphatidylcholine plasmalogen, phosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylethanolamine plasmalogen, phosphatidylserine, sphingomyelin, phosphatidylinositol, phosphatidylglycerol, cardiolipin, and an uncharacterized phosphatide that accounts for 1.5%-3.5% of the detected phospholipids. Metabolic indices, comprised of individual or grouped metabolites, were calculated to further compare and contrast metabolites and to provide pathway-specific metabolic interrelations for each set of phospholipids from cornea and sclera. Significant differences exist between the corneal stroma and the sclera in 9 of the 12 phospholipids, whereas differences exist between the whole cornea and the sclera in 7 of the 12 phospholipids.
作者使用31P核磁共振（NMR）对猪角膜和巩膜的磷脂进行了比较。这些组织构成了眼睛的纤维膜（外层）。由于巩膜与角膜不同，没有上皮或内皮，因此对这些组织的比较分析包括对有上皮和内皮的角膜以及无上皮和内皮的角膜进行检查。定量的磷脂包括：磷脂酰胆碱、溶血磷脂酰胆碱、磷脂酰胆碱缩醛磷脂、磷脂酰乙醇胺、溶血磷脂酰乙醇胺、磷脂酰乙醇胺缩醛磷脂、磷脂酰丝氨酸、鞘磷脂、磷脂酰肌醇、磷脂酰甘油、心磷脂，以及一种未鉴定的磷脂，其占检测到的磷脂的1.5%-3.5%。计算由单个或成组代谢物组成的代谢指数，以进一步比较和对比代谢物，并为来自角膜和巩膜的每组磷脂提供特定途径的代谢相互关系。12种磷脂中有9种在角膜基质和巩膜之间存在显著差异，而12种磷脂中有7种在整个角膜和巩膜之间存在差异。
