College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, People's Republic of China.
J Plant Res. 2010 Sep;123(5):707-14. doi: 10.1007/s10265-009-0290-0. Epub 2009 Dec 18.
Retrotransposons are ubiquitous in the plant kingdom and constitute a large fraction of many plant genomes. Although most retrotransposons from plants were thought to be transcriptionally silent in somatic tissues, evidence of activity under certain conditions is available in some cases. In this study, a complete LTR retrotransposon was isolated from the cultivated strawberry (Fragaria x ananassa) genome using genome walking. The element, named FaRE1, has all the features of a typical Ty1-copia retrotransposon. Its total length was 5,104 bp, comprising a single 3,891 bp open reading frame. It is represented by approximately 96 copies per genome, equivalent to approximately 0.33% of the genome. Transcription of FaRE1 was detected in leaf tissue treated with various phytohormones, such as naphthalene acetic acid, 2,4-dichlorophenoxyacetic acid or abscisic acid . To our knowledge, this is the first report of the isolation of a complete LTR retrotransposon with transcriptional activity in strawberry.
逆转录转座子在植物界中普遍存在，构成了许多植物基因组的很大一部分。尽管大多数来自植物的逆转录转座子在体细胞组织中被认为是转录沉默的，但在某些情况下，有证据表明它们在某些条件下具有活性。在这项研究中，使用基因组步移法从栽培草莓（Fragaria x ananassa）基因组中分离出一个完整的 LTR 逆转录转座子。该元件命名为 FaRE1，具有典型 Ty1-copia 逆转录转座子的所有特征。其全长为 5104bp，包含一个单一的 3891bp 开放阅读框。它在每个基因组中大约有 96 个拷贝，相当于基因组的 0.33%左右。在经过各种植物激素处理的叶片组织中检测到 FaRE1 的转录，如萘乙酸、2,4-二氯苯氧乙酸或脱落酸。据我们所知，这是首次报道在草莓中分离出具有转录活性的完整 LTR 逆转录转座子。
