基于质谱法的胰岛素样生长因子-1（IGF-1）和人生长激素（hGH）分析。

Mass spectrometry-based analysis of IGF-1 and hGH.

作者信息

Thevis Mario, Bredehöft Michael, Kohler Maxie, Schänzer Wilhelm

机构信息

Center for Preventive Doping Research - Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany.

出版信息

Handb Exp Pharmacol. 2010(195):201-7. doi: 10.1007/978-3-540-79088-4_9.

DOI:10.1007/978-3-540-79088-4_9

PMID:20020366

Abstract

Mass spectrometric approaches have been used to determine various peptide hormones in sports drug testing. While insulin-like growth factor-1 (IGF-1) and its synthetic analogs are qualitatively and/or quantitatively measured by liquid chromatography-tandem mass spectrometry after immunoaffinity purification, methods of uncovering doping rule violations with illegal applications of human growth hormone (hGH) have not yet been established using mass spectrometry-based assays. However, substantial information on the heterogeneity of hGH, splice variants and post-translational modifications with respective locations as elucidated by mass spectrometry are of utmost importance for improving currently employed immunological procedures.

摘要

质谱方法已被用于体育药物检测中各种肽类激素的测定。虽然胰岛素样生长因子-1（IGF-1）及其合成类似物在免疫亲和纯化后通过液相色谱-串联质谱进行定性和/或定量测定，但尚未建立基于质谱分析的方法来发现使用非法应用人生长激素（hGH）的兴奋剂违规行为。然而，通过质谱阐明的关于hGH的异质性、剪接变体和翻译后修饰及其各自位置的大量信息对于改进目前使用的免疫学方法至关重要。