Department of Physiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey.
Toxicol Mech Methods. 2008 Jan;18(9):705-10. doi: 10.1080/15376510801897705.
ABSTRACT The present study was conducted using 6 to 8 month old New Zealand white male rabbits (nine rabbits per treatment group). Daily gavages of 3, 1.5, 0.75, or 0 mg endosulfan/kg BW in corn oil resulted in the death of five (55%), three (33%), zero (0%), and zero (0%) rabbits, respectively, in 30 days. All rabbits were monitored for any observable toxic symptoms throughout the experimental period (30 d) and they also were weighed weekly to monitor body weight gain. All deaths occurred within the first 3 weeks and nervous symptoms were observed only for a few minutes before death. Alterations recorded in hematological parameters within the groups (hemoglobin, packed cell volume, and total erythrocyte count) were due to endosulfan exposure. Serum alkaline phosphatase (ALP) and aspartate aminotransferase (AST) levels were significantly elevated in the 3 mg/kg dose group. Gross post-mortem and histopathological changes in various organs (lung, liver, kidney, and testes) of rabbits treated with endosulfan were observed with typical organochlorine dose-dependent signs of toxicity. Although some animals appeared to adjust to relatively high daily doses of endosulfan for 30 days, biochemical and histological evidence indicated varied liver and kidney damage relative to dosage administered to these animals. The current subacute (30 day) study suggested a NOAEL of 0.75 mg endosulfan/kg in New Zealand white rabbits.
摘要 本研究使用 6 至 8 月龄新西兰雄性白兔（每组 9 只）进行。每天灌胃 3、1.5、0.75 或 0mg 硫丹/kgBW 玉米油，结果在 30 天内分别有 5 只（55%）、3 只（33%）、0 只（0%）和 0 只（0%）兔子死亡。所有兔子在整个实验期间（30 天）均监测到任何可观察到的毒性症状，并每周称重以监测体重增加。所有死亡均发生在第 3 周内，死前仅观察到几分钟的神经症状。各组记录的血液学参数变化（血红蛋白、红细胞压积和总红细胞计数）归因于硫丹暴露。血清碱性磷酸酶（ALP）和天冬氨酸氨基转移酶（AST）水平在 3mg/kg 剂量组显著升高。用硫丹处理的兔子的各种器官（肺、肝、肾和睾丸）的大体尸检和组织病理学变化，观察到具有典型有机氯毒性剂量依赖性的变化。尽管一些动物似乎能够适应 30 天相对较高的每日硫丹剂量，但生化和组织学证据表明，与给予这些动物的剂量相比，肝脏和肾脏受到不同程度的损伤。本亚急性（30 天）研究表明，新西兰白兔的硫丹无观察到不良作用水平（NOAEL）为 0.75mg/kg。
