饮用水给予六价铬对感染登革热病毒的雄性小鼠脾脏的影响。

Effects of dengue virus infection on the spleen of male mice given hexavalent chromium with drinking water.

机构信息

Biomembrane Division, Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, 226001India.

出版信息

Toxicol Mech Methods. 2005;15(5):323-9. doi: 10.1080/153765291009732.

PMID:20021051

Abstract

The present study was undertaken to investigate the effects of dengue virus (DV) infection in male mice given drinking water containing 250 ppm Cr (VI) and the normal control male mice given plain water to drink. On the basis of intake of water in 24 h, the average dose of Cr (VI) in each mouse was 14.8 mg/kg. After 3, 6, and 9 weeks of drinking Cr (VI), a set of five mice from each group were inoculated intracerebrally (ic) with a 1000 x LD(50) (100 times the lethal dose that kills 50% mice) dose of DV, and the effects on the spleen were studied at the fourth and eightth day postinoculation. It was observed that Cr (VI) drinking and DV infection led to reduction in the weight of the spleen, but the peak reduction was seen in Cr (VI)-fed mice infected with DV, being 30, 34, and 61% at 3, 6, and 9 weeks respectively. A similar response was seen with respect to the cytotoxic activity of spleen homogenates, phagocytic activity of macrophages, and the mitogenic response of spleen cells to concanavalin A from different groups of animals, being most marked (58 to 60%) at the ninth week of Cr (VI) drinking. This shows a summation of adverse effects of DV infection in mice preexposed to Cr (VI).

摘要

本研究旨在探讨感染登革热病毒（DV）对饮用含 250ppmCr（VI）水的雄性小鼠和饮用普通水的正常雄性对照小鼠的影响。基于 24 小时的饮水量，每只小鼠平均摄入 Cr（VI）剂量为 14.8mg/kg。在饮用 Cr（VI）3、6 和 9 周后，每组 5 只小鼠分别经脑内（ic）接种 1000×LD（50）（100 倍致死剂量，可杀死 50%的小鼠）剂量的 DV，并在接种后第 4 和第 8 天研究对脾脏的影响。结果观察到，Cr（VI）摄入和 DV 感染导致脾脏重量减轻，但在感染 DV 的 Cr（VI）喂养小鼠中，这种减轻最为明显，分别在第 3、6 和 9 周减少了 30%、34%和 61%。在脾匀浆的细胞毒性活性、巨噬细胞的吞噬活性和脾细胞对刀豆蛋白 A 的有丝分裂反应方面，各组动物也表现出类似的反应，在 Cr（VI）摄入的第 9 周最为显著（58%至 60%）。这表明，预先暴露于 Cr（VI）的小鼠感染 DV 后会产生累加的不利影响。