Department of Biophysics, Punjab University, Chandigarh, India.
Toxicol Mech Methods. 2005;15(6):419-23. doi: 10.1080/15376520500194775.
The neurotoxic effects of lead are age-related phenomena, which in turn are dependent on nutrition status as well as the presence of other micronutrients. The present study was designed to study the protective effects of selenium on rat pups exposed to lead. The activities of different enzymes in cerebrum and cerebellum regions were estimated following maternal lead neurotoxicity through placenta. The parental animals were exposed to different treatments for 5 weeks prior to gestation and 3 weeks during gestation; that is, animals were exposed for a total period of 8 weeks and rat pups obtained from exposed parents were allowed to survive on their mothers' milk for a period of 1 month, after which they were sacrificed. Activities of succinic dehydrogenase (SDH), acetylcholine estrase (AChE), and Na(+)/K(+)-ATPase were studied in the cerebrum and cerebellum of the rat pups at 4 weeks of age. A significant reduction in the activity of all the three enzymes-SDH, AChE and Na(+)/K(+) -ATPase-was observed in the lead-treated group, except in the case of AChE, which increased in the case of cerebrum. However, changes in different enzyme activities were less pronounced in the animals that were treated simultaneously with selenium and lead. No pups were delivered to the mothers who were exposed to selenium alone for period of 8 weeks.
铅的神经毒性作用是与年龄相关的现象，而这又取决于营养状况以及其他微量元素的存在。本研究旨在研究硒对胎盘暴露于铅的大鼠幼仔的保护作用。通过胎盘对母体铅神经毒性作用，估计大脑和小脑区域的不同酶的活性。在妊娠前和妊娠期间，亲代动物接受了不同的处理，总共接受了 8 周的处理，然后从暴露于铅的亲代动物中获得的幼仔可以通过母亲的乳汁生存 1 个月，然后将其处死。在 4 周龄时，研究了大脑和小脑中琥珀酸脱氢酶（SDH）、乙酰胆碱酯酶（AChE）和 Na(+)/K(+) -ATP 酶的活性。在铅处理组中，除了大脑中的 AChE 增加外，所有三种酶（SDH、AChE 和 Na(+)/K(+) -ATP 酶）的活性均显著降低，但同时用硒和铅处理的动物中的变化不那么明显。在单独暴露于硒 8 周的母亲中，没有幼仔分娩。
