Plant Genetic Division, Nuclear Institute of Agriculture (NIA), Tando Jam, 70060, Pakistan.
J Integr Plant Biol. 2009 Dec;51(12):1080-5. doi: 10.1111/j.1744-7909.2009.00880.x.
The present study aimed to quantify the methyl esters of lenoleic acid (LA), gamma-lenolenic acid (LNA) and oleic acid (OL) in the oil of Brassica napus mutants. Five stable mutants (ROO-75/1, ROO-100/6, ROO-125/12, ROO-125/14, and ROO-125/17) of B. napus cv. 'Rainbow' (P) and three mutants (W97-95/16, W97-0.75/11 and W97-.075/13) of B. napus cv. 'Westar' (P) at M6 stage, exhibiting better yield and yield components, were analyzed for essential fatty acids. The highest seed yield was observed in the mutant (ROO-100/6) followed by ROO-125/14 of Rainbow, that is, 34% and 32% higher than their parent plants, respectively. Westar mutant W97-75/11 also showed 30% higher seed yield than its parent plant. High performance liquid chromatography analysis of the composition of fatty acids indicated that OL was the most dominant fatty acid, ranging from 39.1 to 66.3%; LA was second (15.3-41.6%) and LNA was third (18.1-28.9%). Mutant ROO-125/14 showed higher OL contents than parent (Rainbow). These results are expected to support the approval of ROO-125/14 in the National Uniform Varietal Yield Trials (NUVYT) as a new variety based on high oil quality.
本研究旨在定量分析油菜突变体油中的亚油酸（LA）、γ-亚麻酸（LNA）和油酸（OL）的甲酯。对 5 个稳定的油菜突变体（ROO-75/1、ROO-100/6、ROO-125/12、ROO-125/14 和 ROO-125/17）（P 型）和 3 个油菜突变体（W97-95/16、W97-0.75/11 和 W97-0.075/13）（P 型）在 M6 期进行了分析，这些突变体表现出更好的产量和产量构成，分析了必需脂肪酸。在突变体（ROO-100/6）中观察到最高的种子产量，其次是 Rainbow 的 ROO-125/14，分别比其亲本高 34%和 32%。Westar 突变体 W97-75/11 的种子产量也比其亲本高 30%。脂肪酸组成的高效液相色谱分析表明，OL 是最主要的脂肪酸，范围为 39.1%至 66.3%；LA 位居第二（15.3%至 41.6%），LNA 位居第三（18.1%至 28.9%）。突变体 ROO-125/14 比亲本（Rainbow）表现出更高的 OL 含量。这些结果有望支持 ROO-125/14 在国家统一品种产量试验（NUVYT）中作为一种高油质新品种获得批准。
