[中国浙江省麻疹疫苗株与野生株基因组的比较分析]
[Comparative analysis of measles genome between vaccine strain and wild-type strain in Zhejiang province of China].
作者信息
Xu Chang-ping, Yan Ju-ying, Feng Yan, Mao Hai-yan, Lu Yi-yu
机构信息
Center for Disease Control and Prevention of Zhejiang Province, Key Laboratory of Emergence Detection for Public Health of Zhejiang Province, Hangzhou 310051, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2009 Aug;43(8):723-6.
OBJECTIVE
To explore the distinction between wild-type strain MVi/Zhejiang, CHN/7.05/4 and vaccine strain Shanghai-191 at genome level.
METHODS
After sequencing of measles wild-stain MVi/Zhejiang. CHN/7.05/4, the distinction between the wild-type strain and the vaccine strain was analysed by MEGA 3.1 software at genome level, and the antigen variation was studied by means of combining the epidemiological data.
RESULTS
There were 822 nucleotide differences (5.17%) and 161 amino acid differences between these two strains, including three glycosylation sites variation found. Meanwhile, the antigen ratio between wild-type strain and vaccine strain was found to be 5.66.
CONCLUSION
There should be certain differences between the contemporary wild-type strain MVi/Zhejiang, CHN/7.05/4 and vaccine strain Shanghai-191 at genome level, and the protective effects of measles vaccine should be studied further.
目的
在基因组水平上探究野生型毒株MVi/浙江株、CHN/7.05/4株与疫苗株上海-191株之间的差异。
方法
对麻疹野毒株MVi/浙江株、CHN/7.05/4株进行测序后，利用MEGA 3.1软件在基因组水平分析野生型毒株与疫苗株之间的差异，并结合流行病学数据研究抗原变异情况。
结果
这两株毒株之间存在822个核苷酸差异（5.17%）和161个氨基酸差异，包括发现三个糖基化位点变异。同时，野生型毒株与疫苗株之间的抗原比为5.66。
结论
当代野生型毒株MVi/浙江株、CHN/7.05/4株与疫苗株上海-191株在基因组水平上应存在一定差异，麻疹疫苗的保护效果需进一步研究。
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