Wu Wan-xin, Zhang Yan-ping, Wen Xiao-wei, Lu Ning, Tang Zheng-ying, Zhang Yi, Wang Zhen, Wang Wei, Yang Hong-jie
Department of Pathology, First Affiliated Hospital of Jiaxing College, Jiaxing 314000, China.
Zhonghua Bing Li Xue Za Zhi. 2009 Aug;38(8):519-23.
To evaluate the relationship between epithelial-mesenchymal transition and basal cell-like phenotype breast cancer (BLBC).
Three hundred and eighty two cases of breast cancers including basal cell-like, luminal A, luminal B and Her-2 subtypes were collected from 458 cases of invasive breast cancers based on their immunophenotypes. They were then stained immunohistochemically with FOXC-2, vimentin, Syndecan-1 and E-cadherin. The relationship of these markers with the basal cell-like phenotype of breast cancer was studied.
Of the 41 BLBC cases, FOXC-2, vimentin and Syndecan-1 were positive in 14 cases (34.1%), 18 cases (43.9%) and 36 cases (87.7%) respectively; E-cadherin expression was reduced in 26 cases (63.4%). The positive rates of FOXC-2 and vimentin were higher in BLBC than in other subtypes of breast cancer (P < 0.01). The expression of E-cadherin was the lowest among the 4 subtypes of breast cancers (P < 0.01). Syndecan-1 was positive in the stromal cells adjacent to cancer cells in 17 cases (41.5%) BLBC and the expression was higher than that in other subtypes (P = 0.007). There existed a correlation between FOXC-2 and vimentin expression in BLBC (r = 0.607, P < 0.01). The rates of positive lymph nodes in FOXC-2 and vimentin positive BLBC cases were 71.4% and 66.7% respectively, and both were higher than those of FOXC-2 and vimentin negative BLBC cases (P = 0.002 and P = 0.001).
Epithelial-mesenchymal transition is probably related to the basal cell-like phenotype of breast cancers, and this may be one of the reasons accounting for the different biological behavior of BLBC from other subtypes of breast cancer.
评估上皮-间质转化与基底样表型乳腺癌（BLBC）之间的关系。
基于免疫表型，从458例浸润性乳腺癌中收集了382例乳腺癌，包括基底样、腔面A型、腔面B型和Her-2亚型。然后用FOXC-2、波形蛋白、Syndecan-1和E-钙黏蛋白进行免疫组织化学染色。研究这些标志物与乳腺癌基底样表型的关系。
在41例BLBC病例中，FOXC-2、波形蛋白和Syndecan-1分别有14例（34.1%）、18例（43.9%）和36例（87.7%）呈阳性；26例（63.4%）E-钙黏蛋白表达降低。BLBC中FOXC-2和波形蛋白的阳性率高于其他亚型乳腺癌（P < 0.01）。E-钙黏蛋白的表达在4种乳腺癌亚型中最低（P < 0.01）。17例（41.5%）BLBC中与癌细胞相邻的基质细胞中Syndecan-1呈阳性，其表达高于其他亚型（P = 0.007）。BLBC中FOXC-2与波形蛋白表达之间存在相关性（r = 0.607，P < 0.01）。FOXC-2和波形蛋白阳性的BLBC病例中阳性淋巴结率分别为71.4%和66.7%，均高于FOXC-2和波形蛋白阴性的BLBC病例（P = 0.002和P = 0.001）。
上皮-间质转化可能与乳腺癌的基底样表型有关，这可能是BLBC与其他亚型乳腺癌生物学行为不同的原因之一。
