Jin Xin, Qian Jun, Yu Da-Jun, Guo Chen-Xu, Li Nan, Zhu Qing, Zhao Yan
Department of Surgical Oncology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233003,China.
Department of Pathology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233003,China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 Mar;49(2):215-220.
To investigate the expression of FOXC-2,YB-1 and related proteins and their influences on development,invasion and metastases in gastric carcinoma.
A total of 193 tissue samples were collected,including 50 cases of normal gastric mucosa, 50 cases of gastric mucosal intraepithelial neoplasia and 93 cases of primary gastric carcinoma. The 93 cases of primary gastric carcinoma included 74 cases of positive lymph node metastasis tissues, 19 cases of nonmetastasis tissues and 33 cases of distant metastasis tissues. Immuohistochemistry was used to detect the expression and distribution of FOXC-2,YB-1,E-cadherin, Vimentin and MMP-2 in normal and intraepithelial neoplasia,gastric carcinoma,positive lymph node metastasis tissues and distant metastasis tissues.
The expressions of FOXC-2,YB-1,Vimentin and MMP-2 in gastric carcinoma were significantly higher than those in normal and intraepithelial neoplasia while the expression of E-cadherin was significantly lower (<0.05).The expressions of FOXC-2,YB-1 were significantly correlated with low expression of E-cadherin and high expression of Vimentin and MMP-2 (<0.05). The expression of FOXC-2 protein was significantly correlated with TNM stage,lymph node metastasis and distant metastases (<0.05).The expression of YB-1 protein was significantly correlated with TNM stage,differentiation degree,invasion depth,lymph node metastasis and distant metastasis (<0.05). The expression of MMP-2 protein was closely related to the degree of differentiation,invasion depth,lymph node metastasis and distant metastasis (<0.05).
FOXC-2, YB-1 may be related to the occurrence, development, invasion and metastasis of gastric carcinoma. The possible mechanism is to promote the invasion and metastasis of cancer cells by activating the epithelial mesenchymal transition process and up regulating the expression of MMP-2.
探讨FOXC-2、YB-1及相关蛋白的表达及其对胃癌发生、侵袭和转移的影响。
收集193例组织样本，包括50例正常胃黏膜、50例胃黏膜上皮内瘤变和93例原发性胃癌。93例原发性胃癌中包括74例阳性淋巴结转移组织、19例无转移组织和33例远处转移组织。采用免疫组织化学法检测正常及上皮内瘤变、胃癌、阳性淋巴结转移组织和远处转移组织中FOXC-2、YB-1、E-钙黏蛋白、波形蛋白和基质金属蛋白酶-2（MMP-2）的表达及分布。
胃癌组织中FOXC-2、YB-1、波形蛋白和MMP-2的表达明显高于正常及上皮内瘤变组织，而E-钙黏蛋白的表达明显降低（<0.05）。FOXC-2、YB-1的表达与E-钙黏蛋白低表达、波形蛋白和MMP-2高表达显著相关（<0.05）。FOXC-2蛋白表达与TNM分期、淋巴结转移和远处转移显著相关（<0.05）。YB-1蛋白表达与TNM分期、分化程度、浸润深度、淋巴结转移和远处转移显著相关（<0.05）。MMP-2蛋白表达与分化程度、浸润深度、淋巴结转移和远处转移密切相关（<0.05）。
FOXC-2、YB-1可能与胃癌的发生、发展、侵袭和转移有关。可能机制是通过激活上皮-间质转化过程并上调MMP-2的表达促进癌细胞的侵袭和转移。
