Microbiology Department, Hospital Universitari de Bellvitge, IDIBELL, University of Barcelona, Feixa Llarga, s/n 08907 Hospitalet de Llobregat, Barcelona, Spain.
Diagn Microbiol Infect Dis. 2010 Mar;66(3):308-13. doi: 10.1016/j.diagmicrobio.2009.10.018.
Activity of tigecycline against nosocomial secondary peritonitis isolates collected along 18 months in 29 Spanish hospitals was tested by Etest in a central laboratory, considering Food and Drug Administration (FDA)/British Society for Antimicrobial Chemotherapy (BSAC)/European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints. A total of 600 facultative/aerobic isolates (392 Gram negative, 208 Gram positive) and 100 anaerobes were tested. None of the 220 Escherichia coli isolates was resistant to tigecycline (MIC(50)/MIC(90) = 0.25/0.5 microg/mL), with 0.5% (FDA breakpoint) and 3.6% (BSAC/EUCAST breakpoint) intermediate strains. All Extended-spectrum beta-lactamase (ESBL)-producing E. coli isolates (15 strains), all Klebsiella pneumoniae, and Klebsiella oxytoca isolates (42 strains) were susceptible to tigecycline. No isolates resistant to tigecycline were found among Streptococcus viridans, Staphylococcus aureus, and Enterococcus faecium, but 18.9% of Enterococcus faecalis strains were intermediate following BSAC/EUCAST breakpoints. All (but 1) isolates of the Bacteroides fragilis group (n = 45) were tigecycline susceptible, as well as Gram-positive anaerobes. Tigecycline offers an adequate activity profile against isolates from secondary peritonitis when tested by Etest regardless of the breakpoints used for categorization.
在一个中心实验室，通过 Etest 对 29 家西班牙医院在 18 个月内收集的医院获得性继发性腹膜炎分离株进行了替加环素活性测试，考虑了食品和药物管理局 (FDA)/英国抗菌化疗学会 (BSAC)/欧洲抗菌药物敏感性测试委员会 (EUCAST) 折点。共测试了 600 株需氧/兼性厌氧菌分离株（392 株革兰氏阴性菌，208 株革兰氏阳性菌）和 100 株厌氧菌。220 株大肠埃希菌分离株中无一株对替加环素耐药（MIC(50)/MIC(90) = 0.25/0.5 μg/mL），其中 0.5%（FDA 折点）和 3.6%（BSAC/EUCAST 折点）为中介株。所有产超广谱β-内酰胺酶 (ESBL) 的大肠埃希菌分离株（15 株）、所有肺炎克雷伯菌和产酸克雷伯菌分离株（42 株）均对替加环素敏感。未发现对替加环素耐药的粪肠球菌、屎肠球菌和屎肠球菌，但根据 BSAC/EUCAST 折点，18.9%的粪肠球菌分离株为中介。脆弱拟杆菌组的所有（除 1 株外）分离株（n = 45）和革兰氏阳性厌氧菌均对替加环素敏感。通过 Etest 测试，替加环素对继发性腹膜炎分离株的活性谱在不同的分类折点下均表现出足够的活性。
