从鸵鸟（Struthio camelus）垂体中分离并鉴定促肾上腺皮质激素。
The isolation and characterization of corticotropin from the pituitary gland of the ostrich Struthio camelus.
作者信息
Naudé R J, Oelofsen W
出版信息
Biochem J. 1977 Sep 1;165(3):519-23. doi: 10.1042/bj1650519.
Abstract
- Avian corticotropin (ACTH) was purified from both fresh and aged pituitary glands of the ostrich Struthio camelus. 2. The isolation of corticotropin in pure form involved acid/acetone extraction, NaCl fractionation, CM-cellulose chromatography and Sephadex G-50 chromatography. 3. The hormone preparations from fresh and aged glands behaved as single substances on polyacrylamide-gel electrophoresis, and both preparations were found to consist of 39 amino acid residues, in identical molar proportions for the different amino acids. 4. The isoelectric points of the two hormone preparations were estimated to be in the range pH 8.3-8.7, indicating possible differences in amide content, and the N-terminal amino acid of both preparations appeared to be serine. 5. The hormone preparations from fresh and aged glands exhibited similar biological potencies (73 and 77 i.u./mg respectively), as measured by steroidogenesis in vitro. 6. Apart from possible differences in amide content, the corticotropin preparations obtained from fresh and aged glands appear to be indistinguishable.
摘要
- 从鸵鸟（Struthio camelus）的新鲜和陈旧垂体中纯化出禽促肾上腺皮质激素（ACTH）。2. 以纯形式分离促肾上腺皮质激素涉及酸/丙酮提取、氯化钠分级分离、CM-纤维素色谱法和葡聚糖凝胶G-50色谱法。3. 新鲜和陈旧腺体的激素制剂在聚丙烯酰胺凝胶电泳上表现为单一物质，并且发现两种制剂均由39个氨基酸残基组成，不同氨基酸的摩尔比例相同。4. 两种激素制剂的等电点估计在pH 8.3 - 8.7范围内，表明酰胺含量可能存在差异，并且两种制剂的N端氨基酸似乎都是丝氨酸。5. 通过体外类固醇生成测定，新鲜和陈旧腺体的激素制剂表现出相似的生物活性（分别为73和77国际单位/毫克）。6. 除了酰胺含量可能存在差异外，从新鲜和陈旧腺体获得的促肾上腺皮质激素制剂似乎无法区分。
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