生成和鉴定人δ-珠蛋白特异性单克隆抗体。

Generation and characterization of human delta-globin-specific monoclonal antibodies.

机构信息

Department of Immunology, Southern Medical University, Guangzhou, China.

出版信息

Blood Cells Mol Dis. 2010 Mar 15;44(3):127-32. doi: 10.1016/j.bcmd.2009.11.003. Epub 2009 Dec 21.

DOI:10.1016/j.bcmd.2009.11.003

PMID:20022528

Abstract

The human delta-globin chain, unique to the hemoglobin A2 (HbA2) heterotetramer, is important for the evaluation of hemoglobinopathy. However, there are no well-defined antibodies specific for the delta-globin chain, a fact that is attributed a striking similarity (93%) in amino acid sequence between delta-globin and beta-globin of the hemoglobin A (HbA). In this study, two monoclonal antibodies (mAbs) against the delta-globin chain were generated and designated as 2H4 and 1H11. These antibodies were specific to HbA2 and do not cross-react with HbA and HbF (fetal hemoglobin). Moreover, the expression of HbA2 in fetal liver and mature erythrocytes was determined using these two mAbs. In addition to being useful tools for research or diagnosis, these antibodies could be valuable for development of rapid and effective antibody-based immunoassays of HbA2 expression in erythroid cells and non-erythroid tissue.

摘要

人类的 δ-珠蛋白链是血红蛋白 A2（HbA2）异四聚体所特有的，对于评估血红蛋白病很重要。然而，目前还没有针对 δ-珠蛋白链的特异性抗体，这一事实归因于 δ-珠蛋白和血红蛋白 A（HbA）的β-珠蛋白之间氨基酸序列（93%）的惊人相似性。在这项研究中，生成了两种针对 δ-珠蛋白链的单克隆抗体（mAb），并分别命名为 2H4 和 1H11。这些抗体特异性针对 HbA2，与 HbA 和 HbF（胎儿血红蛋白）不发生交叉反应。此外，使用这两种 mAb 测定了胎肝和成熟红细胞中 HbA2 的表达。除了作为研究或诊断的有用工具外，这些抗体对于开发快速有效的基于抗体的免疫分析方法来检测红细胞和非红细胞组织中 HbA2 的表达也很有价值。