评价一株问号钩端螺旋体 Canicola 血清型重组 LigB 蛋白在牛钩端螺旋体病血清学诊断酶联免疫吸附试验中的应用。
Evaluation of a recombinant LigB protein of Leptospira interrogans serovar Canicola in an enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis.
机构信息
Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar 243122, Uttar Pradesh, India.
出版信息
Res Vet Sci. 2010 Jun;88(3):375-8. doi: 10.1016/j.rvsc.2009.11.004. Epub 2009 Dec 22.
A recombinant leptospiral lipoprotein, LigB, was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLigB IgG ELISA). The standard reference test (Microscopic agglutination test, MAT) of 200 serum samples from cattle suspected of leptospirosis showed that 95 (47.5%) samples had positive agglutination titres, which ranged from 100 to 1600. In rLigB IgG ELISA, 49% of the samples were positive. Sensitivity of IgG ELISA for 95 bovine sera, which had MAT titres of greater than or equal to 100, were 100%. ELISA showed a specificity of 97.1% with 105 bovine sera, which were negative at a 1:50 dilution in MAT for Leptospira interrogans serovars. The results of ELISA and MAT correspond very good. When analytical specificity of IgG ELISA was evaluated using bovine serum samples from animals showing the serum antibodies to other pathogens, no cross-reaction was observed. Thus the recombinant LigB IgG ELISA can be used instead of the MAT as an aid to the diagnosis of bovine leptospirosis.
一种重组钩端螺旋体脂蛋白 LigB 经酶联免疫吸附试验（rLigB IgG ELISA）评估，可用于诊断牛钩端螺旋体病。对 200 份疑似感染钩端螺旋体病牛血清进行标准参考测试（显微镜凝集试验，MAT），结果显示 95（47.5%）份血清的凝集效价呈阳性，范围从 100 到 1600。在 rLigB IgG ELISA 中，有 49%的样本呈阳性。对 MAT 效价大于或等于 100 的 95 份牛血清进行 IgG ELISA 的敏感性为 100%。ELISA 对 105 份 MAT 稀释度为 1：50 呈阴性的牛血清的特异性为 97.1%。ELISA 和 MAT 的结果非常吻合。当使用显示对其他病原体血清抗体的牛血清样本评估 IgG ELISA 的分析特异性时，未观察到交叉反应。因此，重组 LigB IgG ELISA 可替代 MAT 用于辅助诊断牛钩端螺旋体病。
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