优化 RNA 提取方法，用于 PCR 定量检测芳香族化合物降解基因。
Optimization of RNA extraction for PCR quantification of aromatic compound degradation genes.
机构信息
Department of Agronomy, Purdue University, West Lafayette, IN 47907-2054, USA.
出版信息
Appl Environ Microbiol. 2010 Feb;76(4):1282-4. doi: 10.1128/AEM.01939-09. Epub 2009 Dec 18.
Abstract
Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.
摘要
采用 7 种不同的细菌菌株和引物对以及混合群落来评估逆转录定量 PCR (Q-PCR)和 Q-PCR 氧合酶基因在监测污染场地生物修复中的各种方法。比较不同的 RNA 提取试剂盒时，观察到最大活性存在差异。
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