Lazo O, Singh A K, Singh I
Department of Pediatrics, Medical University of South Carolina, Charleston 29425.
J Neurochem. 1991 Apr;56(4):1343-53. doi: 10.1111/j.1471-4159.1991.tb11431.x.
We analyzed the postnatal peroxisome development in rat brain by measuring the enzyme activities of catalase and acyl-CoA oxidase and beta-oxidation of [1-14C]lignoceric acid. These enzyme activities were higher between 10 and 16 days of postnatal life and then decreased. We developed and compared two different methods for isolation of enriched peroxisomes from 10-day-old rat brain by using a combination of differential and density gradient centrifugation techniques. Peroxisomes in Percoll (self-generating gradient) banded at a density of 1.036 +/- 0.012 g/ml and in Nycodenz continuous gradient at 1.125 +/- 0.014 g/ml. Acyl-CoA oxidase, D-amino acid oxidase, L-pipecolic acid oxidase, and dihydroxyacetone phosphate acyltransferase activities and activities for the oxidation of very long chain fatty acid (lignoceric acid) were almost exclusively associated with catalase activity (a marker enzyme for peroxisomes) in the gradient. The postnatal increase in peroxisomal activity with the onset of myelination and the presence of enzyme for the biosynthesis of plasmalogens and oxidation of very long chain fatty acid (both predominant constituents of myelin) suggest that brain peroxisomes may play an important role in the assembly and turnover of myelin.
我们通过测量过氧化氢酶和酰基辅酶A氧化酶的酶活性以及[1-14C]木蜡酸的β-氧化，分析了大鼠脑内出生后过氧化物酶体的发育情况。这些酶活性在出生后10至16天之间较高，然后下降。我们开发并比较了两种不同的方法，通过结合差速离心和密度梯度离心技术，从10日龄大鼠脑中分离富集的过氧化物酶体。在Percoll（自生成梯度）中，过氧化物酶体在密度为1.036±0.012 g/ml处形成条带，在Nycodenz连续梯度中在1.125±0.014 g/ml处形成条带。酰基辅酶A氧化酶、D-氨基酸氧化酶、L-哌啶酸氧化酶和二羟丙酮磷酸酰基转移酶活性以及极长链脂肪酸（木蜡酸）氧化活性几乎完全与梯度中的过氧化氢酶活性（过氧化物酶体的标记酶）相关。随着髓鞘形成开始，过氧化物酶体活性在出生后增加，并且存在用于缩醛磷脂生物合成和极长链脂肪酸氧化（髓鞘的两种主要成分）的酶，这表明脑过氧化物酶体可能在髓鞘的组装和更新中起重要作用。
