Institute of Biochemistry and Molecular Biology, Binzhou Medical University, 264003 Yantai, Shandong, People's Republic of China.
Ir J Med Sci. 2010 Jun;179(2):217-23. doi: 10.1007/s11845-009-0418-1. Epub 2009 Dec 20.
The CT120 gene had been proven to be a novel gene closely related to pulmonary carcinogenesis and cancer progression. Our aim was to explore the mechanism of growth suppression caused by silencing CT120.
CT120 was detected in lung cancer tissues and the cell line A549, and the cell clones for silencing CT120 were obtained. Then the target genes were detected and the downstream proteins from the silencing of CT120 were separated and identified.
The expression of CT120 was higher in lung cancer tissues and A549 cells. Silencing of CT120 was shown to inhibit cell growth, reduce the expression of cyclin D1 and Cdk4, and increase the expression of p53 and caspase-3. The differential proteins were related to carcinogenesis, invasiveness, and metastasis.
CT120 may play an important role in tumor progression, and the down-regulation of CT120 expression could be a new drug target candidate in the treatment of lung cancer.
CT120 基因已被证明是与肺肿瘤发生和癌症进展密切相关的新型基因。我们的目的是探讨沉默 CT120 引起生长抑制的机制。
检测 CT120 在肺癌组织和细胞系 A549 中的表达情况，并获得沉默 CT120 的细胞克隆。然后检测靶基因，并分离和鉴定沉默 CT120 的下游蛋白。
CT120 在肺癌组织和 A549 细胞中的表达水平较高。沉默 CT120 可抑制细胞生长，降低 cyclin D1 和 Cdk4 的表达，增加 p53 和 caspase-3 的表达。差异蛋白与致癌、侵袭和转移有关。
CT120 可能在肿瘤进展中发挥重要作用，下调 CT120 的表达可能成为治疗肺癌的新药物靶点候选物。
