Université de Rennes1, Molecular and Cellular Interactions UMR6026, Hip, IFR140 GFAS, Bâtiment 13, Campus de Beaulieu, 35042 Rennes Cedex, France.
Prog Biophys Mol Biol. 2010 Jan;102(1):16-37. doi: 10.1016/j.pbiomolbio.2009.12.007. Epub 2009 Dec 16.
The rate of transcription initiation is the main level of quantitative control of gene expression, primarily responsible for the accumulation of mRNAs in the cell. Many, if not all, molecular actors involved in transcription initiation are known but the mechanisms underlying the frequency of initiations, remain elusive. To make the connection between transcription factors and the frequency of transcription initiation, intricated aspects of this complex activity are classified i) depending on whether or not the DNA-bound transcription factors directly activate the commitment to transcription and ii) on the destructive or non-destructive effect of transcription initiation on the stability of promoter complexes. Two possible sources of synergy allowing the combinatorial specificity of transcription factors action are compared, for binding to DNA and for recruiting transcription machineries. Tentative formulations are proposed to discriminate the different micro-reversible modes of DNA binding cooperativity modulating the specificity and dosage of transcription initiation.
转录起始的速率是基因表达定量控制的主要水平，主要负责细胞中 mRNA 的积累。尽管并非所有，但许多参与转录起始的分子因素已被确定，但启动频率的机制仍然难以捉摸。为了将转录因子与转录起始的频率联系起来，将这种复杂活动的复杂方面分类为：i）取决于 DNA 结合的转录因子是否直接激活转录的承诺，以及 ii）转录起始对启动子复合物稳定性的破坏或非破坏性影响。比较了允许转录因子作用的组合特异性的两种可能的协同作用源，一种是用于 DNA 结合，另一种是用于募集转录机制。提出了试探性的公式来区分调节转录起始的特异性和剂量的 DNA 结合协同作用的不同微观可逆模式。
