大豆低聚糖的化学成分分析及其对高血脂大鼠 ATP 酶活性的影响。

Chemical composition analysis of soybean oligosaccharides and its effect on ATPase activities in hyperlipidemic rats.

机构信息

Department of Emergency, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

出版信息

Int J Biol Macromol. 2010 Mar 1;46(2):229-31. doi: 10.1016/j.ijbiomac.2009.12.005. Epub 2009 Dec 16.

DOI:10.1016/j.ijbiomac.2009.12.005

PMID:20025901

Abstract

A water-soluble oligosaccharide was isolated from the water extract of soybean, which is widely grown worldwide due to its nutritional benefits of a full range of amino acids and a high protein content. In this study, we analysed chemical composition of soybean oligosaccharides (SBOS) using high performance liquid chromatography (HPLC). The results obtained were compared with the following sugar standards: mannose, galactose, xylose, arabinose, fucose and glucose. Results showed that SBOS was composed of galactose (65.3%), mannose (15.6%), fructose (7.8%) and glucose (8.7%). The pharmacological action of SBOS has been studied in hyperlipidemic rats fed high-fat diet. In hyperlipidemic rats, feeding with SBOS (150 and 300 mg/kg b.w.) exerted a pharmacological effect as assessed by reversal of plasma levels of lactic acid, nitric oxide (NO), vascular endothelial growth factor (VEGF) levels, and heart Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase activities. These results indicated that SBOS may have beneficial effects on the therapy of some heart diseases, such as coronary artery diseases.

摘要

一种水溶性低聚糖从大豆的水提取物中分离出来，由于其富含各种氨基酸和高蛋白质含量，在全世界范围内广泛种植。在本研究中，我们使用高效液相色谱（HPLC）分析了大豆低聚糖（SBOS）的化学成分。将得到的结果与以下糖标准品进行比较：甘露糖、半乳糖、木糖、阿拉伯糖、岩藻糖和葡萄糖。结果表明，SBOS 由半乳糖（65.3%）、甘露糖（15.6%）、果糖（7.8%）和葡萄糖（8.7%）组成。SBOS 的药理学作用已在高脂饮食喂养的高血脂大鼠中进行了研究。在高血脂大鼠中，用 SBOS（150 和 300mg/kg bw）喂养表现出药理学作用，如血浆乳酸、一氧化氮（NO）、血管内皮生长因子（VEGF）水平以及心脏 Na+-K+-ATP 酶、Ca2+-ATP 酶和 Mg2+-ATP 酶活性的逆转。这些结果表明，SBOS 可能对某些心脏病（如冠状动脉疾病）的治疗具有有益作用。