Institute of Biomedicine, Pharmacology, University of Helsinki, PO Box 63, FI-00014, Helsinki, Finland.
World J Gastroenterol. 2009 Dec 28;15(48):6068-74. doi: 10.3748/wjg.15.6068.
To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.
Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC x GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.
Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).
IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.
通过比较肠易激综合征(IBS)患者与健康对照者的全球黏膜代谢谱,探讨 IBS 的病理生理学。
本研究纳入了 15 名符合罗马 II 标准的 IBS 患者和 9 名健康志愿者。采用脂质组学(UPLC/MS)和代谢组学(GC x GC-TOF)联合方法,对升结肠黏膜活检的进行全局代谢谱分析。
总体而言,IBS 患者的脂质水平升高。促炎溶血磷脂酰胆碱的上调最为显著。IBS 患者中上调明显的其他脂质组包括脂毒性神经酰胺、糖脂和二酰基和三酰基甘油。在代谢物中,与健康受试者相比,IBS 患者的环状酯 2(3H)-呋喃酮上调了近 14 倍(P = 0.03)。
IBS 黏膜的特点是具有独特的促炎和脂毒性代谢谱。特别是,几种脂质物质如溶血磷脂和神经酰胺的含量增加。