可裂解连接子用于光交联小分子亲和基质。

Cleavable linker for photo-cross-linked small-molecule affinity matrix.

机构信息

Tohoku University, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

出版信息

Bioconjug Chem. 2010 Jan;21(1):182-6. doi: 10.1021/bc900316q.

PMID:20028022

Abstract

The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.

摘要

在光活化连接子中引入可裂解位点，可通过非选择性碳烯加成/插入反应将小分子固定在亲和基质上，这使得验证亲和基质上固定的小分子的存在成为可能。它还允许对共价结合到固定小分子上的蛋白质进行高效检测。