Forrester D M, Van Rensburg C E, Anderson R
Department of Immunology, University of Pretoria, Republic of South Africa.
Mutat Res. 1991 Mar;247(1):39-44. doi: 10.1016/0027-5107(91)90031-i.
Dithranol at therapeutic concentrations (5-40 micrograms ml-1) induced strand breaks in human leucocyte DNA in vitro in a dose-related manner. Leucocytes from individuals with chronic granulomatous disease (CGD) incurred substantially less DNA strand breaks than did normal leucocytes during exposure to dithranol indicating that activated phagocytes are involved. H-7, 4-beta-bromophenacyl bromide (BPB) and staurosporine, all inhibitors of protein kinase C, decreased both dithranol-mediated activation of the phagocyte respiratory burst and induction of DNA strand breaks. Similar effects were observed with the hydrogen peroxide scavenger catalase. These results suggest that dithranol induces DNA strand breaks, mainly as a result of pro-oxidative interactions with phagocytes.
治疗浓度（5 - 40微克/毫升）的地蒽酚在体外以剂量相关的方式诱导人白细胞DNA链断裂。在接触地蒽酚期间，慢性肉芽肿病（CGD）患者的白细胞发生的DNA链断裂明显少于正常白细胞，这表明活化的吞噬细胞参与其中。蛋白激酶C的所有抑制剂H - 7、4 - β - 溴苯甲酰溴（BPB）和星形孢菌素，均可降低地蒽酚介导的吞噬细胞呼吸爆发激活以及DNA链断裂的诱导。过氧化氢清除剂过氧化氢酶也观察到类似的效果。这些结果表明，地蒽酚诱导DNA链断裂，主要是与吞噬细胞发生促氧化相互作用的结果。
