Nagahara A, Ohshita K, Nasuno S
Research and Development Division, Kikkoman Corporation, Chiba, Japan.
Mutat Res. 1991 Mar;262(3):171-6. doi: 10.1016/0165-7992(91)90018-y.
Soy sauce pretreated with 2300 ppm nitrite caused no more aberrations than did untreated soy sauce in the chromosomal aberration test in vitro using a Chinese hamster fibroblast cell line with or without S9 mixture. The aberration induction by soy sauce is likely to be caused by the 17% sodium chloride it contains. Soy sauce with or without pretreatment with 2300 ppm nitrite was orally given to ICR mice at a dose of 14 ml/kg body weight once or 6 ml/kg body weight/day for 5 consecutive days. This oral administration did not induce any significant increase in micronuclei in the micronucleus test in vivo.
在使用中国仓鼠成纤维细胞系并添加或不添加S9混合物的体外染色体畸变试验中，用2300 ppm亚硝酸盐预处理的酱油所引起的畸变并不比未处理的酱油更多。酱油诱导畸变可能是由其所含的17%氯化钠引起的。将用或不用2300 ppm亚硝酸盐预处理的酱油以14 ml/kg体重的剂量一次性给予ICR小鼠，或以6 ml/kg体重/天的剂量连续5天给予。这种口服给药在体内微核试验中未引起微核的任何显著增加。
