[从动物粪便和异胸木蜂分离出的培养放线菌的多样性及生物活性]
[Diversity and bioactivities of cultured actinomycete isolated from animal feces and Xylocopa dissimilis].
作者信息
Jiang Yi, Cao Yanru, Cai Xiangfeng, Xu Lihua, Jiang Chenglin
机构信息
Yunnan Institute of Microbiology, The National Engineering Center for Research of Microbial Pharmaceuticals, Yunnan University, Kunming 650091, China.
出版信息
Wei Sheng Wu Xue Bao. 2009 Sep;49(9):1152-7.
OBJECTIVE
To study the diversity and bioactivities of cultured actinomycete from feces of Ailuropoda melanoleuca, Equus burchelli and Rhizomys sinensis, and Xylocopa dissimilis body.
METHODS
Fresh feces samples of animals' and Xylocopa dissimilis were collected. We used four media to isolate the actinomycete. Strains were identified by phylogenetic analysis of 16S rRNA gene sequences. Hydrolization and enzyme activities of isolates were examined.
RESULTS
Actinomycetes (121) from the samples were isolated on four media. Among them 47 strains belonged to 9 actinomycetes genera. Six known rare genera and one possible new genus of actinomyctes from Rhizomys sinensis were identified. Three possible new species from other three samples were identified. Some strains could hydrolyze chicken feather and cellulose.
CONCLUSION
It is considered that the coprophilous microorganisms not only have important function on the digestion and absorption of animal feed, but also are important resource for development of industrial products, including enzyme and bioactive metabolites.
目的
研究大熊猫、细纹斑马、中华竹鼠粪便及黑胸木蜂躯体中培养的放线菌的多样性及生物活性。
方法
采集动物新鲜粪便样本及黑胸木蜂样本。使用四种培养基分离放线菌。通过16S rRNA基因序列的系统发育分析鉴定菌株。检测分离株的水解和酶活性。
结果
在四种培养基上从样本中分离出121株放线菌。其中47株属于9个放线菌属。鉴定出中华竹鼠的6个已知稀有属和1个可能的新放线菌属。鉴定出其他三个样本中的3个可能的新物种。一些菌株能够水解鸡毛和纤维素。
结论
认为嗜粪微生物不仅在动物饲料的消化吸收中具有重要作用，而且是包括酶和生物活性代谢物在内的工业产品开发的重要资源。
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