[静脉注射脂肪组织来源干细胞在软组织损伤SD大鼠体内的迁移]
[Migration of intravenously injected adipose tissue-derived stem cells in SD rats with soft tissue wound].
作者信息
Nan Hua, Gao Jian-Hua, Lu Feng, Liao Yun-Jun
机构信息
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2009 Sep;25(5):372-6.
OBJECTIVE
To explore the migration of intravenously injected adipose tissue-derived stem cells (ADSCs) in SD rats with soft tissue wound.
METHODS
1.8 cm x 1.8 cm full thickness skin defect and 0.5 cm in depth soft tissue defects were made on the back of 6 SD rats. ADSCs were isolated and cultured in vitro for 3 passages. 2.4 x 10(6) ADSCs were labeled with DiI and transplanted into the SD rats through tail vein. Normal skin and wound tissue samples were collected for fluorescent distribution observation 24, 48 days after injection, respectively.
RESULTS
Compared to normal skin, more fluorescent positive cells were detected in the margin and deep layer of the wound 24 days after operation. But it is accumulated within dermis and adenoid 48 days after operation.
CONCLUSION
Wound can probably induce the migration and accumulation of intravenously transplanted ADSCs.
目的
探讨静脉注射脂肪组织来源干细胞（ADSCs）在软组织损伤的SD大鼠体内的迁移情况。
方法
在6只SD大鼠背部制作1.8 cm×1.8 cm全层皮肤缺损及深度为0.5 cm的软组织缺损。将ADSCs分离并体外培养3代。将2.4×10(6)个用DiI标记的ADSCs经尾静脉注入SD大鼠体内。分别在注射后24、48天采集正常皮肤和伤口组织样本观察荧光分布。
结果
术后24天，与正常皮肤相比，在伤口边缘及深层检测到更多荧光阳性细胞。但术后48天，其聚集于真皮层及腺样组织内。
结论
伤口可能诱导静脉移植的ADSCs发生迁移和聚集。
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