[通过实时聚合酶链反应鉴定人类致病性天花病毒和猴痘病毒]

[Identification of human pathogenic variola and monkeypox viruses by real-time polymerase chain reaction].

作者信息

Kostina E V, Gavrilova E V, Riabinin V A, Shchelkunov S N, Siniakov A N

出版信息

Vopr Virusol. 2009 Nov-Dec;54(6):28-33.

PMID:20030279

Abstract

A kit of specific oligonucleotide primers and hybridization probes has been proposed to detect orthopoxviruses (OPV) and to discriminate human pathogenic viruses, such as variola virus and monkey virus by real-time polymerase chain reaction (PCR). For real-time PCR, the following pairs of fluorophore and a fluorescence quencher were used: TAMRA-BHQ2 for genus-specific probes and FAM-BHQ1 for species-specific ones (variola virus, monkeypox virus, ectomelia virus). The specificity of this assay was tested on 38 strains of 6 OPV species and it was 100%.

摘要

已提出一套特异性寡核苷酸引物和杂交探针，用于通过实时聚合酶链反应（PCR）检测正痘病毒（OPV），并区分人类致病病毒，如天花病毒和猴痘病毒。对于实时PCR，使用了以下荧光团和荧光淬灭剂对：用于属特异性探针的TAMRA-BHQ2和用于种特异性探针（天花病毒、猴痘病毒、兔痘病毒）的FAM-BHQ1。该检测方法在6种OPV物种的38个菌株上进行了特异性测试，结果为100%。