[162例儿童急性淋巴细胞白血病中HLA - DRB1*15与发病机制的关系]

[Relation of HLA-DRB1*15 with pathogenesis in 162 childhood cases of acute lymphoblastic leukemia].

作者信息

Wang Xiao-Jing, Ai Xiao-Fei, Sun Hai-Yan, Wang Fang, Xu Shi-Cai, Gui Fu-Min, Shen Hui, Wang Zhi-Qiang, Li Qing-Hua, Liang Xiao-Lan, Zhu Xiao-Fan

机构信息

Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Scinces & Peking Union Medical College, Tianjin 300020, China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Dec;17(6):1507-10.

PMID:20030936

Abstract

To unravel the relation of HLA-DRB115 with childhood acute lymphoblastic leukemia (ALL), 162 childhood patients with ALL were selected for this investigation. 1 000 normal umbilical cord blood samples were used as control.HLA-DRB115 and HLA-DRB5* were typed by polymerase chain reaction (PCR) analysis. The relation of HLA-DRB115 with childhood ALL was studied by calculating the chi-square test and relative risk. The results showed that the antigen frequencies and allele frequencies of HLA-DRB115 in childhood patients with ALL were 40.12% and 22.62% respectively, while the antigen frequencies and allele frequencies of HLA-DRB115 in control were 30.80% and 16.81% respectively, there were significant difference between them (chi(2) = 5.560, p = 0.018, RR = 1.506). In conclusion, the antigen frequencies and allele frequencies of HLA-DRB115 in childhood patients with ALL were higher than those in control, so the HLA-DRB1*15 gene is one of the genetic risk factors for childhood ALL. These preliminary data may be useful for further study on the pathogenesis of childhood ALL.

摘要

为了阐明HLA - DRB115与儿童急性淋巴细胞白血病（ALL）之间的关系，本研究选取了162例儿童ALL患者。1000份正常脐带血样本用作对照。采用聚合酶链反应（PCR）分析对HLA - DRB115和HLA - DRB5进行分型。通过计算卡方检验和相对风险来研究HLA - DRB115与儿童ALL的关系。结果显示，儿童ALL患者中HLA - DRB115的抗原频率和等位基因频率分别为40.12%和22.62%，而对照组中HLA - DRB115的抗原频率和等位基因频率分别为30.80%和16.81%，两者之间存在显著差异（χ² = 5.560，p = 0.018，RR = 1.506）。综上所述，儿童ALL患者中HLA - DRB115的抗原频率和等位基因频率高于对照组，因此HLA - DRB115基因是儿童ALL的遗传危险因素之一。这些初步数据可能有助于进一步研究儿童ALL的发病机制。