Du Ying, Liang Xiao-lan, Li Qian, Wu Wen-jie, Liu Jian, Sun Le-jing, Qiu Lu-gui
Union Stem Cell & Gene Engineering Co. Ltd., Tianjin, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Apr;21(2):285-8. doi: 10.7534/j.issn.1009-2137.2013.02.005.
This study was aimed to investigate the correlation between HLA gene distribution and allele frequency of the patients with leukemia. PCR-SSP technique was used to detect the HLA genotype of 2994 umbilical cord blood units from healthy newborns (as control), the detecting result of which was compared with HLA genotypes of 1246 patients with leukemia searched in our cord blood bank. The differences between two groups were compared and analyzed. The results indicated that as compared with the control group, the allele frequencies of HLA-B56 (0.56%), B70 (0.24%) obviously increased (RR = 2.2546, 6.2598, χ(2) = 5, 5.98, P < 0.05), while the allele frequencies of HLA-A03 (3.45%), A30 (4.86%), B13 (8.75%), B44 (3.25%), B61* (5.70%), DRB107 (8.23%), DRB115 (14.21%) obviously decreased in patients with leukemia (RR = 0.5889, 0.7187, 0.7359, 0.5713, 0.7127, 0.6242, 0.7976, χ(2) = 19.23, 9.82, 14.33, 20.48, 11.99, 33.21, 11.56, P < 0.01). It is concluded that HLA-B56, B70 alleles seem to be characterized by the genetic susceptibility to leukemia and may be served as risk markers for leukemia occurrence, while the HLA-A03, A30, B13, B44, B61, DRB107, DRB1*15 can be considered as genetic indicators for resistance of leukemia.
本研究旨在探讨白血病患者人类白细胞抗原（HLA）基因分布与等位基因频率之间的相关性。采用聚合酶链反应-序列特异性引物（PCR-SSP）技术检测2994例健康新生儿脐带血（作为对照）的HLA基因型，并将检测结果与本脐血库中检索到的1246例白血病患者的HLA基因型进行比较。比较并分析两组之间的差异。结果表明，与对照组相比，白血病患者HLA-B56（0.56%）、B70（0.24%）等位基因频率明显升高（RR = 2.2546, 6.2598, χ(2)=5, 5.98, P < 0.05），而HLA-A03（3.45%）、A30（4.86%）、B13（8.75%）、B44（3.25%）、B61*（5.70%）、DRB107（8.23%）、DRB115（14.21%）等位基因频率明显降低（RR = 0.5889, 0.7187, 0.7359, 0.5713, 0.7127, 0.6242, 0.7976, χ(2)=19.23, 9.82, 14.33, 20.48, 11.99, 33.21, 11.56, P < 0.01）。结论：HLA-B56、B70等位基因似乎具有白血病遗传易感性特征，可能作为白血病发生的风险标志物，而HLA-A03、A30、B13、B44、B61、DRB107、DRB1*15可被视为白血病抗性的遗传指标。
