New Jersey Institute of Technology, Physics Department, Newark, 07102, USA.
Biophys Chem. 2010 Feb;146(2-3):133-7. doi: 10.1016/j.bpc.2009.11.005. Epub 2009 Nov 30.
The presence of the resting membrane potential has a strong effect on the dielectric behavior of cell suspensions. Using this observation and a well-established theoretical model, the low frequency dielectric dispersion curves of E. coli cell suspensions are de-convoluted to obtain the resting membrane potential of E. coli cells at various growth stages. Four regions of the exponential growth stage are investigated and the measurements indicate that the membrane depolarizes from -220mV in the early exponential phase to -140mV in the late exponential phase. The conductivity of the cell suspension is also found to decrease as the cells progress from the early to the late exponential phases.
静息膜电位的存在对细胞悬浮液的介电行为有很强的影响。利用这一观察结果和一个成熟的理论模型，对大肠杆菌细胞悬浮液的低频介电色散曲线进行反卷积，以获得不同生长阶段大肠杆菌细胞的静息膜电位。研究了指数生长阶段的四个区域，测量结果表明，膜电位从早期指数期的-220mV 去极化到晚期指数期的-140mV。还发现，随着细胞从早期指数期向晚期指数期的发展，细胞悬浮液的电导率也降低。
