肿瘤内皮标志物 7 的分泌型和膜型转录本在骨肉瘤中的优先表达。
Preferential expression of the secreted and membrane forms of tumor endothelial marker 7 transcripts in osteosarcoma.
机构信息
Department of Orthopedic Research, Mayo Clinic, Experimental Pathology, Rochester, MN 55905, USA.
出版信息
Anticancer Res. 2009 Nov;29(11):4317-22.
BACKGROUND
High expression of tumor endothelial marker 7 (TEM7) is correlated with osteogenic sarcoma (OS) metastasis and poor survival of patients. The TEM7 gene produces four alternatively spliced transcripts with distinct functional domains; the expression pattern of these transcripts in OS is unknown.
MATERIALS AND METHODS
mRNA expression was assessed in 5 OS cell lines, 7 normal bone, and 9 OS tumor specimens by reverse transcriptase polymerase chain reaction.
RESULTS
All OS cell lines, 6/9 tumors but none of the bone specimens expressed mRNA of TEM7 secreted forms 1 and 2. A total of 3/5 OS cell lines, 8/9 of tumors and 4/7 of bone specimens expressed mRNA of the TEM7 intracellular form. One out of 5 cell lines, 2/7 tumors and none of the bone specimens expressed mRNA of the TEM7 membrane form. The secreted forms had 20-fold higher expression in metastatic (LM7) compared to non-metastatic (SAOS-2) cells.
CONCLUSION
The mRNA of secreted and the membrane forms of TEM7 are preferentially expressed in OS.
背景
肿瘤内皮标志物 7（TEM7）的高表达与骨肉瘤（OS）的转移和患者的不良生存相关。TEM7 基因产生四个具有不同功能域的选择性剪接转录本；这些转录本在 OS 中的表达模式尚不清楚。
材料与方法
通过逆转录聚合酶链反应，在 5 个骨肉瘤细胞系、7 个正常骨和 9 个骨肉瘤肿瘤标本中评估 mRNA 表达。
结果
所有骨肉瘤细胞系、9 个肿瘤中的 6 个，但没有一个骨标本表达 TEM7 分泌形式 1 和 2 的 mRNA。共有 3/5 的骨肉瘤细胞系、8/9 的肿瘤和 4/7 的骨标本表达 TEM7 细胞内形式的 mRNA。5 个细胞系中的 1 个、7 个肿瘤中的 2 个和没有骨标本表达 TEM7 膜形式的 mRNA。分泌形式在转移性（LM7）细胞中比非转移性（SAOS-2）细胞高 20 倍表达。
结论
TEM7 的分泌形式和膜形式的 mRNA 在骨肉瘤中优先表达。
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