在体外研究DR - 3438、替尼酸、茚达立酮和呋塞米的肝毒性。

Hepatotoxicity of DR-3438, tienilic acid, indacrinone and furosemide studied in vitro.

作者信息

Takagi S, Takayama S, Onodera T

机构信息

Drug Safety Research Center, Daiichi Seiyako Co., Ltd., Tokyo, Japan.

出版信息

Toxicol Lett. 1991 Mar;55(3):287-93. doi: 10.1016/0378-4274(91)90009-u.

DOI:10.1016/0378-4274(91)90009-u

PMID:2003271

Abstract

A new diuretic antihypertensive, DR-3438, and marketed diuretic drugs were evaluated for their toxicity in vitro. Hepatocytes were isolated from male rats by the collagenase perfusion method and incubated in Dulbecco's modified Eagle medium containing various doses of DR-3438, tienilic acid, indacrinone or furosemide. Tienilic acid decreased cell viability and glutathione content in hepatocytes and increased lipid peroxidation in the cells. Indacrinone also decreased cell viability, but neither cell viability nor glutathione content was affected by furosemide or DR-3438.

摘要

对一种新型利尿降压药DR - 3438以及市售利尿药进行了体外毒性评估。采用胶原酶灌注法从雄性大鼠中分离肝细胞，并将其在含有不同剂量DR - 3438、替尼酸、茚达立酮或呋塞米的杜尔贝科改良伊格尔培养基中孵育。替尼酸降低了肝细胞的活力和谷胱甘肽含量，并增加了细胞内的脂质过氧化。茚达立酮也降低了细胞活力，但呋塞米或DR - 3438对细胞活力和谷胱甘肽含量均无影响。